ab117122 reacts strongly with gp41, gp120 and gp160 in western blot assays. ELISA based peptide mapping studies have confirmed high titer reactivity to HIV MN env peptide in the following regions: (6332)IEPLGVAPTKAKRRV, (6360) CTTTVPWNASWSNKS and moderate activity to multiple other regions on the env polypeptide.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Predicted molecular weight: 97 kDa.
Use at an assay dependent dilution.
Endoproteolytic processing of human immunodeficiency virus type 1 (HIV1) gp160 membrane glycoprotein precursor into gp120 and gp41 is necessary for formation of infectious HIV particles.
The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.