Overview

  • Product nameAnti-HIV1 gp120 antibody
    See all HIV1 gp120 primary antibodies
  • Description
    Goat polyclonal to HIV1 gp120
  • SpecificityRecognises gp120. Antiserum does not cross-react with human T or B cells.
  • Tested applicationsSuitable for: Conjugation, ELISA, WBmore details
  • Immunogen

    Purified native gp120 from strain IIIB.

Properties

Applications

Our Abpromise guarantee covers the use of ab21179 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Conjugation Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
WB 1/1000. Predicted molecular weight: 120 kDa. Dilution optimised using Chromogenic detection

Target

  • RelevanceHIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.
  • Cellular localizationEnvelope surface glycoprotein.
  • Alternative names
    • Glycoprotein 120 antibody
    • gp120 antibody
    • SU antibody
    • Surface protein antibody

References for Anti-HIV1 gp120 antibody (ab21179)

This product has been referenced in:

See all 6 Publications for this product

Product Wall

Laboratory has finally confirmed that these products cannot be supplied with Texas Red conjugation so I am sorry we are unable to honour your request this time.

We however have conjugation kits available, e.g.

http://www.abcam.com/E...

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Thank you for contacting us. Theoretically your outline makes sense. However, we have not tested the products this way and cannot guarantee that the binding sites of the antibodies will not interfere with each other. I hope this is helpful. Please le m...

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Thank you very much for your reply.

Please keep me updated about the results after making those changes. I'll be happy to send another GP120 antibody or any other antibody that you might need.

I look forward to hearing from you. If ...

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Thank you very much for your email and for keeping me updated about the results with the recombinant protein.

I am sorry to see that the results are still not as expected. From your images, it seems that the bands in both of the brain lysates...

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Thank you very much for your replies yesterday.

I'm sending a free of charge vial of ab69717 on the order *** which should arrive tomorrow. This protein has been tested in Western blot using 0.1-1 ug per lane. Please keep me updated about the...

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Thank you very much for your reply.

I can send a vial of one of our GP120 proteins to use as a positive control. Would ab69717 work for you?

http://www.abcam.com/HIV1-gp120-protein-ab69717.html

Please confirm this and I wi...

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Thank you very much for your call today and for letting us know about the trouble with these antibodies.

As we discussed, please keep me updated about the results after trying the antibodies with the recombinant protein. We do fully guarantee...

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Unfortunately, it looks like we do not have the unconjugated form of ab53837 Anti-HIV1 antibody (HRP) in our catalogue.
However, we have one antibody which may interest you:

http://www.abcam.com/index.html?datasheet=21179 (or use the follo...

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Thank you for your enquiry and your interest in our products. I have conducted a search for you on our website and found several antibodies against HIV gp120. Although, none of them have been specifically listed for use in affinity purification (AP...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (10 ul Supernatant from PBMC culture containing vir)
Loading amount 10 cells
Specification 10 ul Supernatant from PBMC culture containing vir
Gel Running Conditions Non-reduced Denaturing
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Username

Mr. Hoku West-Foyle

Verified customer

Submitted Nov 06 2009

1-10 of 13 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"