Anti-HIV1 gp120 antibody (HRP) (ab53840)

Overview

  • Product nameAnti-HIV1 gp120 antibody (HRP)
    See all HIV1 gp120 primary antibodies
  • Description
    Goat polyclonal to HIV1 gp120 (HRP)
  • ConjugationHRP
  • SpecificityGp120 is the predominant band in WB.
  • Tested applicationsSuitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with HIV1.
  • Immunogen

    Full length native protein, from strain IIIB.

  • General notes

    Estimated molar HRP:IgG substitution is 2-3. Free enzyme is absent.

Properties

Applications

Our Abpromise guarantee covers the use of ab53840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20 - 1/200.
ELISA 1/200 - 1/1000.
IHC-Fr 1/20 - 1/200.

Target

  • RelevanceHIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.
  • Cellular localizationEnvelope surface glycoprotein.
  • Alternative names
    • Glycoprotein 120 antibody
    • gp120 antibody
    • SU antibody
    • Surface protein antibody

References for Anti-HIV1 gp120 antibody (HRP) (ab53840)

ab53840 has not yet been referenced specifically in any publications.

Product Wall

Application Western blot
Loading amount 100000 cells
Gel Running Conditions Reduced Denaturing (3-8% Tris-acetate SDS-PAGE)
Sample Human Cell lysate - other (HOS)
Specification HOS
Treatment transfected with HIV-1 Env expression plasmid
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

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Submitted Oct 17 2014

Thank you for your enquiry. These 4 products (ab21179, ab20105, ab20262, ab20008) were produced from the same goat antiserum. Catalog ab21179 is the purified antibody. The purified antibody is then labeled to produce the other catalog numbers: Bio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"