Overview

  • Product name
    Anti-HIV1 gp120 antibody (HRP)
    See all HIV1 gp120 primary antibodies
  • Description
    Goat polyclonal to HIV1 gp120 (HRP)
  • Conjugation
    HRP
  • Specificity
    Gp120 is the predominant band in WB.
  • Tested applications
    Suitable for: WB, ELISA, IHC-Frmore details
  • Species reactivity
    Reacts with HIV1.
  • Immunogen

    Full length native protein, from strain IIIB.

  • General notes

    Estimated molar HRP:IgG substitution is 2-3. Free enzyme is absent.

Properties

Applications

Our Abpromise guarantee covers the use of ab53840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/20 - 1/200.
ELISA 1/200 - 1/1000.
IHC-Fr 1/20 - 1/200.

Target

  • Relevance
    HIV1 is equipped with the envelope gp160 glycoprotein for interaction with Langerhans cells (LCs) and dendritic cells (DCs), the members of the innate immune system, which confront the virus at the portal of virus entry in the human body. These cells are equipped with receptors by which they bind and endocytose the virus. The gp120 glycoprotein is used for binding to CD4 receptor and CCR5 co-receptor of T helper 2 (Th2) cells, and is able to induce FcepsilonRI(+) hematopoietic cells to produce IL4, which inactivates the host adaptive immune response.
  • Cellular localization
    Envelope surface glycoprotein.
  • Alternative names
    • Glycoprotein 120 antibody
    • gp120 antibody
    • SU antibody
    • Surface protein antibody

References for Anti-HIV1 gp120 antibody (HRP) (ab53840)

This product has been referenced in:
  • Grimm SK  et al. Directed evolution of a yeast-displayed HIV-1 SOSIP gp140 spike protein toward improved expression and affinity for conformational antibodies. PLoS One 10:e0117227 (2015). WB . Read more (PubMed: 25688555) »

See 1 Publication for this product

Product Wall

Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (3-8% Tris-acetate SDS-PAGE)
Sample
Human Cell lysate - other (HOS)
Specification
HOS
Treatment
transfected with HIV-1 Env expression plasmid
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Oct 17 2014

Thank you for your enquiry. These 4 products (ab21179, ab20105, ab20262, ab20008) were produced from the same goat antiserum. Catalog ab21179 is the purified antibody. The purified antibody is then labeled to produce the other catalog numbers: Bio...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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