Overview

  • Product nameAnti-HIV1 gp41 antibody [10E9]
    See all HIV1 gp41 primary antibodies
  • Description
    Mouse monoclonal [10E9] to HIV1 gp41
  • SpecificityReacts with Human Immunodeficiency Virus Type 1(HIV 1) gp41 envelope transmembrane protein. Also exhibits strong reactivity with HIV 1 env gene precursor gp160 using Western blotting.
  • Tested applicationsSuitable for: WB, Radioimmunoprecipitation, ELISA, ICC/IFmore details
  • Immunogen

    Whole HIV viral lysate (B-3 strain). The epitope has not been mapped.

Properties

Applications

Our Abpromise guarantee covers the use of ab9065 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
Radioimmunoprecipitation
ELISA
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution.
    ICC/IF: Use at a concentration of 1 - 10 mg/ml.
    RipA: Use at an assay dependent dilution.
    WB: Use at a concentration of 1 - 10 µg/ml.

    Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevancegp41/120 is the major HIV protein associated with the HIV envelope. It functions as the viral antireceptor or attachment protein. gp41 (or TM) traverses the envelope, whereas gp120 is present on the outer surface and is noncovalently attached to gp41. The precursor of gp120/41 (gp160) is synthesized in the endoplasmic reticulum and is transported via the golgi body to the cell surface. Upon activation of the envelope glycoprotein (gp120/41) by cellular receptors, gp41 undergoes conformational changes that mediate fusion of the viral and cellular membranes.
    • Alternative names
      • Env antibody
      • Glycoprotein 41 antibody
      • gp41 antibody
      • Human Immunodeficiency Virus 1 antibody
      • TM antibody
      • Transmembrane protein antibody
      see all

    References for Anti-HIV1 gp41 antibody [10E9] (ab9065)

    This product has been referenced in:
    • Papsidero LD  et al. Monoclonal antibody identifies a highly conserved and immunodominant epitope of the human immunodeficiency virus transmembrane protein. Hybridoma 7:117-28 (1988). Read more (PubMed: 2453451) »

    See 1 Publication for this product

    Product Wall

    Thank you for your patience in this matter. I have received replies from the originators of ab9065 and ab18633. In regards to ab9065, this antibody was produced to the HIV-1 B-3 strain and its' reactivity with the LAV strain has not been tested in ...

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    At this time we do not have specific information regarding what type of fixatives were used for immunofluorescence staining, but we assume HIV infected cells were paraformaldehyde fixed to slides. They were then developed or stained with ab9065. We are...

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    The immunogen used is a whole HIV viral lysate and the antibody was identified as gp41 by Western blotting. The epitope has not been mapped.

    This antibody has not yet been tested for use with paraffin embedded tissue.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"