Overview

  • Product nameAnti-HIV1 Nef antibody [3F2]
    See all HIV1 Nef primary antibodies
  • Description
    Mouse monoclonal [3F2] to HIV1 Nef
  • Tested applicationsSuitable for: ELISA, WB, Radioimmunoprecipitationmore details
  • Species reactivity
    HIV
  • Immunogen

    Recombinant full length protein

  • EpitopeMapped to amino acids 31-35 (GAASR, HIV-1 HxB2).

Properties

Applications

Our Abpromise guarantee covers the use of ab25866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 23 kDa.
Radioimmunoprecipitation Use at an assay dependent dilution.

Target

  • RelevanceNef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
  • Alternative names
    • C terminal core protein antibody
    • F protein antibody
    • Nef antibody
    • Negative factor antibody
    • p27 antibody
    see all

Anti-HIV1 Nef antibody [3F2] images

  • Lane 1 : Anti-HIV1 Nef antibody [3F2] (ab25866)
    Lane 2 :

    Lane 1 : Recombinant HIV1 Nef (B subtype)
    Lane 2 : Protein size marker


    Predicted band size : 23 kDa

References for Anti-HIV1 Nef antibody [3F2] (ab25866)

This product has been referenced in:
  • Kjerrström A  et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). Read more (PubMed: 11352667) »
  • Collings A  et al. Humoral and cellular immune responses to HIV-1 nef in mice DNA-immunised with non-replicating or self-replicating expression vectors. Vaccine 18:460-7 (1999). Read more (PubMed: 10519935) »

See all 6 Publications for this product

Product Wall

Application Western blot
Sample Human Cell lysate - whole cell (HEK293T cells)
Gel Running Conditions Reduced Denaturing (4-12% gel)
Loading amount 10 µg
Specification HEK293T cells
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Dr. Jason Lee

Verified customer

Submitted Aug 15 2016

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"