Overview

  • Product nameAnti-HIV1 Nef antibody
    See all HIV1 Nef primary antibodies
  • Description
    Rabbit polyclonal to HIV1 Nef
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with HIV1.
  • Immunogen

    Recombinant Nef protein over-expressed in E. coli with a plasmid carrying the Nef coding region of HIV1 virus, subtype B.

  • Positive control
    • Extract of MT4 cells uninfected or infected with HIV1 (LAI strain).

Properties

Applications

Our Abpromise guarantee covers the use of ab63918 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Predicted molecular weight: 24 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceNef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
  • Alternative names
    • C terminal core protein antibody
    • F protein antibody
    • Nef antibody
    • Negative factor antibody
    • p27 antibody
    see all

Anti-HIV1 Nef antibody images

  • All lanes : Anti-HIV1 Nef antibody (ab63918) at 1/1000 dilution

    Lane 1 : Extract of MT4 cells
    Lane 2 : Extract of MT4 cells infected with HIV1 (LAI strain)


    Predicted band size : 24 kDa
    Observed band size : 27 kDa (why is the actual band size different from the predicted?)

References for Anti-HIV1 Nef antibody (ab63918)

ab63918 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"