Overview

  • Product name
    Anti-HIV1 Nef antibody [JR6]
    See all HIV1 Nef primary antibodies
  • Description
    Mouse monoclonal [JR6] to HIV1 Nef
  • Specificity
    This antibody recognizes the rNef of HIV 1 subtypes A, B and C. It reacts with HIV1 Nef of JR-CSF, BRU, IIIB, LAV & BH10 strains.
  • Tested applications
    Suitable for: WB, ELISA, ICC/IFmore details
  • Species reactivity
    HIV1
  • Immunogen

    Recombinant Nef protein of HIV1 (B subtype consensus).

  • Epitope
    Mapped to amino acids 195-205 (ARELHPEYYKD - B subtype consensus)
  • General notes
    Also known as clone 01-009.

Properties

Applications

Our Abpromise guarantee covers the use of ab42358 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
ICC/IF
  • Application notes
    ELISA: 1/25000.
    ICC/IF: Use at an assay dependent dilution.
    RipA: Use at an assay dependent dilution.
    WB: 1/5000. Predicted molecular weight: 24 kDa.

    Titer in ELISA/WB using rNEF of HIV 1 Subtype A.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Relevance
      Nef is a early protein that appears to play a role in optimizing the host cell environment for viral replication without causing cell death by apoptosis. Nef enhances virus infectivity and pathogenicity. It down modulates surface MHC I molecules and internalized molecules are sequested to the trans-Golgi network. The number of cell surface CD4 antigen are decreased by interacting with the Src family kinase LCK thereby inducing LCK CD4 dissociation and by increasing clathrin-dependent endocytosis of this antigen to target it to lysosomal degradation.
    • Alternative names
      • C terminal core protein antibody
      • F protein antibody
      • Nef antibody
      • Negative factor antibody
      • p27 antibody
      see all

    Images

    • Lane 1 : Anti-HIV1 Nef antibody [JR6] (ab42358)
      Lane 2 :

      Lane 1 : Recominant HIV1 Nef (B subtype)
      Lane 2 : Protein size marker


      Predicted band size : 24 kDa

    References

    This product has been referenced in:
    • Lee JH  et al. HIV-Nef and ADAM17-Containing Plasma Extracellular Vesicles Induce and Correlate with Immune Pathogenesis in Chronic HIV Infection. EBioMedicine 6:103-113 (2016). Read more (PubMed: 27211553) »
    • Kjerrström A  et al. Interactions of single and combined human immunodeficiency virus type 1 (HIV-1) DNA vaccines. Virology 284:46-61 (2001). Read more (PubMed: 11352667) »

    See all 7 Publications for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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