Anti-HIV1 p15 antibody (ab66951)
Key features and details
- Rabbit polyclonal to HIV1 p15
- Suitable for: WB, ELISA
- Reacts with: Human immunodeficiency virus
- Isotype: IgG
Overview
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Product name
Anti-HIV1 p15 antibody -
Description
Rabbit polyclonal to HIV1 p15 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
Recombinant p15 protein (HIV1) expressed in E.coli with a plasmid carrying the Gag p15 coding region of HIV1 virus.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab66951 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa).
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ELISA |
Use at an assay dependent dilution.
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Notes |
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WB
1/1000. Detects a band of approximately 15 kDa (predicted molecular weight: 15 kDa). |
ELISA
Use at an assay dependent dilution. |
Target
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Relevance
HIV1 p15(Gag) and its protease cleavage products, nucleocapsid protein p7 and p6, are believed to play a major role in viral infectivity and assembly during the early and late stages of retroviral life cycle. p15 is frequently recognized by HIV1-specific CD8+T cells in natural HIV1 infection and may be an important target for the design of future HIV1 vaccines. -
Cellular localization
Cytoplasmic and Nuclear -
Database links
- Entrez Gene: 155030 Human immunodeficiency virus
- SwissProt: P03366 Human immunodeficiency virus
- SwissProt: P04591 Human immunodeficiency virus
- SwissProt: P18802 Human immunodeficiency virus
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Alternative names
- gag antibody
- Gag polyprotein antibody
- Human Immunodeficiency virus 1 antibody
see all
Images
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All lanes : Anti-HIV1 p15 antibody (ab66951) at 1/1000 dilution
Lane 1 : Molecular weight markers
Lane 2 : Extract of MT4 cells
Lane 3 : Extract of MT4 cells infected with HIV1
(LAI strain)
Predicted band size: 15 kDa
Observed band size: 15 kDa
Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.
Datasheets and documents
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Datasheet download
References (3)
ab66951 has been referenced in 3 publications.
- Lu B et al. Delivering SaCas9 mRNA by lentivirus-like bionanoparticles for transient expression and efficient genome editing. Nucleic Acids Res N/A:N/A (2019). PubMed: 30759231
- Lyu P et al. Delivering Cas9/sgRNA ribonucleoprotein (RNP) by lentiviral capsid-based bionanoparticles for efficient 'hit-and-run' genome editing. Nucleic Acids Res 47:e99 (2019). PubMed: 31299082
- Garcia-Miranda P et al. Stability of HIV Frameshift Site RNA Correlates with Frameshift Efficiency and Decreased Virus Infectivity. J Virol 90:6906-17 (2016). PubMed: 27194769