Overview

  • Product name
    Anti-HIV1 p17 antibody [2D11]
    See all HIV1 p17 primary antibodies
  • Description
    Mouse monoclonal [2D11] to HIV1 p17
  • Specificity
    Reacts with Human Immunodeficiency Virus Type 1 (HIV 1) p17 protein
  • Tested applications
    Suitable for: Flow Cyt, WB, ELISA, ICC/IF, Other, Radioimmunoprecipitationmore details
  • Immunogen

    Other Immunogen Type corresponding to HIV1 p17. ab9067 was raised by immunizing BALB/c mice with purified HIV-1 (Strain IIIB) lysate.

  • Epitope
    Conformational epitope (i.e., pepscan negative)
  • General notes

    The antibody may be used in indirect immunostaining techniques to detect HIV-1 core protein in fresh or cultured HIV-1 infected cells. Studies on core antigen synthesis and metabolism are performed using Western blotting or radioimmunoprecipitation analysis. The antibody may also be valuable for the affinity isolation of HIV-1 core protein.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    Ammonium Sulphate Precipitation
  • Purification notes
    Purified from serum-free culture supernatant.
  • Primary antibody notes
    The antibody may be used in indirect immunostaining techniques to detect HIV-1 core protein in fresh or cultured HIV-1 infected cells. Studies on core antigen synthesis and metabolism are performed using Western blotting or radioimmunoprecipitation analysis. The antibody may also be valuable for the affinity isolation of HIV-1 core protein.
  • Clonality
    Monoclonal
  • Clone number
    2D11
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9067 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. PubMed: 22589721ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 - 10 µg/ml.
ELISA Use at an assay dependent concentration.
ICC/IF Use a concentration of 1 - 10 µg/ml.
AP Use at an assay dependent concentration.
Other Use at an assay dependent concentration.
Radioimmunoprecipitation Use at an assay dependent concentration.

Target

  • Relevance
    HIV1 p17 is the matrix protein of the Gag polyprotein which performs highly complex orchestrated tasks during the assembly, budding, maturation, and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.
  • Cellular localization
    Matrix protein of HIV1 Gag polyprotein
  • Alternative names
    • HIV1 matrix protein p17 antibody
    • HIV1 p17 antibody
    • MA antibody
    • Matrix protein p17 antibody

Images

  • ab9067 staining HIV1 p17 (red) in Jurkat cells by Flow Cytometry. Cells were fixed with paraformaldehyde and permeabilized. The sample was incubated with the primary antibody (1/100 in PBS + 10% FBS) for 30 minutes at 4°C. An undiluted phycoerythrin-conjugated goat anti-mouse IgG was used as the secondary antibody. Gating Strategy: Live Cells.

    See Abreview

  • Flow cytometric analysis of monocyte-derived macrophages infected with HIV, staining HIV1 p17 with ab9067. Cells were treated with 1a,25-dihydroxycholecalciferol (1,25D3) at varying concentrations, before being infected with HIV.
  • Immunofluorescence analysis of Macrophages treated with 100 pM 1,25D3 or vehicle control (EtOH) and infected with HIV1Ba-L, staining HIV1 p17 with ab9067.

    Cells were fixed and permeabilized in PBS supplemented with paraformaldehyde and 0.1% saponin for 30 min. Samples were incubated with primary antibody and an AlexaFluor®488-conjugated goat anti-mouse IgG was used as the secondary antibody.

References

This product has been referenced in:
  • Yaciuk JC  et al. Direct interrogation of viral peptides presented by the class I HLA of HIV-infected T cells. J Virol 88:12992-3004 (2014). Flow Cyt . Read more (PubMed: 25165114) »
  • Campbell GR & Spector SA Vitamin D inhibits human immunodeficiency virus type 1 and Mycobacterium tuberculosis infection in macrophages through the induction of autophagy. PLoS Pathog 8:e1002689 (2012). Flow Cyt . Read more (PubMed: 22589721) »

See all 4 Publications for this product

Customer reviews and Q&As

Application
Flow Cytometry
Fixation
Paraformaldehyde
Sample
Human Cell (Jurkat Cells)
Specification
Jurkat Cells
Gating Strategy
live cells
Permeabilization
Yes - BD FIX/PERM, PERM wash
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Verified customer

Submitted Sep 25 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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