The antibody is used to detect HIV-1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis.
Antibody dilutions should be prepared using buffers containing suitable protein in order to stabilize antibody activity.
Our Abpromise guarantee covers the use of ab9044 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Radioimmunoprecipitation||Use at an assay dependent dilution.|
|WB||Use a concentration of 1 - 10 µg/ml.|
|ELISA||Use at an assay dependent dilution.|
|ICC/IF||Use a concentration of 1 - 10 µg/ml.|