Anti-HIV1 p24 antibody [39/5.4A] (ab9071)

Catalog number(s): 9071
Product(s): Mouse monoclonal [39/5.4A] to HIV1 p24
Survey results
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Telephone section
Q. Did you contact Abcam's Scientific Support by telephone in reference to this complaint?
A. No
Q. Was it clear from the phone options which number you needed to press to get your issue resolved?
A.

Protocol advice section
Q. Did Abcam's Scientific Support give you protocol advice?
A.Yes
Q. How did you feel about being given protocol advice?
A.I was not expecting to be given protocol advice, but was happy to receive this
Q. Did the product work successfully after following the advice?
A.No
Q. Why have you not tried the protocol advice? (Select all that apply.)
A.

Free of charge replacement section
Q. Did Abcam's Scientific Support give you a free of charge replacement?
A. Yes

Q. Did the replacement product work successfully?
A.No
Credit note / refund section
Q. Did Abcam's Scientific Support give you a credit note or a refund?
A.No

Q. Would you have preferred a different outcome from receiving a credit note or refund?
A.
Overall satisfaction section
Q. How satisfied are you with how the complaint was handled?
A.Very satisfied
Q. Could we have done anything to better resolve the problem?
A.
Q. Would you mind if our scientific support team contacted you to better understand what happened and reach an agreeable solution?
A.No, I would not mind
Q. Please share with us any final thoughts you may have about Abcam, the complaints process, or our products.
A.

ANSWER:

Thank you for your participation in Abcam's post complaint survey.

I am sorry that the replacement antibody ab63958 did not work either.

Please let me know what problems you encountered (no bands as before?), and what antibody dilution and incubation times you used. I'd be happy to discuss this further so that we can find an acceptable solution to this problem.

I look forward to hear back from you and assist you further.

WB with p24 from HIV1 expressed in cells
WT and with mutation V86M
sees weak band at 41 and 55 kDa, but no band at 24 kDa
another Ab detects p24, though
loaded 50 ug protein
Ab: 1/500 o/n 4 degree
blocking: BSA or milk
secondary: OK

ANSWER:

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have arranged fora free of charge replacement with ab63958 with our next shipment to Cedarlane.

To check the status of the order please contact Cedarlane by end of this week.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

To whom it may concern,   We have recently purchased Ab400 and ab9071  which come in liquid form. Could you please tell  us the buffer composition?

ANSWER:

Thank you for contacting us.





AB400 is shipped in a liquid form in which the antibody is suspended in a solution containing 0.9% Sodium Chloride and0.1% Sodium Azide (as a preservative).

AB9071is shipped in a liquid form in which the antibody is suspended in PBS.



I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

AB pairing choice
SAMPLE p24 standard solution
PRIMARY ANTIBODY ab9071
DETECTION METHOD enzyme linked
ANTIBODY STORAGE CONDITIONS -20
TYPE OF ELISA
Hi, I have ploughed through the review and Q&A regarding to the paring mAB for sandwich ELISA using HIV-1 p24 as the antigen. I have had AB9071 in house. I wonder whether you have information or may hear any issue if I immobilise ab9072 to pair with AB9071 in the sandwich assay? Thank you.
CP COATING WELL no BLOCKING CONDITIONS no
SECONDARY ANTIBODY ab 9072

ANSWER:

Thank you for contacting us.

I can confirm that anti-HIV1 p24 antibodies ab9071 and ab9072 are suitable for use in performing sandwich ELISAs to detect HIV1 p24. They recognise different epitopes of the protein and have been used previously as a pair for this application.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.

We would like to proceed the sELISA test of HIV1 p24 protein (ab73768) by using Anti-HIV1 p24 antibody [ND1] (ab8246) and Anti-HIV1 p24 antibody [39/5.4A] (ab9071).    I should clarify that the method we used in our experiment is magnetic immunoassay, which detects the magnetic signal by conjugated the detecting antibody with magnetic nanoparticles.    So please send me a discount code if our experiment is qualified. And I will submit the review after our experiment.    Thank you!    Best wishes!

ANSWER:

Indeed, I can confirm that your type of experiments qualifies for the testing discount program.

DISCOUNT CODES: ab73768: 100%ABR-E7TC5 ab8246: 100%ABR-E7TCE ab9071: 100%ABR-E7TCF Expiration date: 09 April 2012

I am very pleased to hear you would like to accept our offer and test these products in sELISA. These codes will give you 1 free primary antibody each (for ab8246 and ab9071) and 1 free protein (for ab73768) before the expiration date. To redeem this offer, please submit an Abreview for your sELISA experiments and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.