Overview

  • Product nameAnti-HIV1 p24 antibody [39/5.4A]
    See all HIV1 p24 primary antibodies
  • Description
    Mouse monoclonal [39/5.4A] to HIV1 p24
  • SpecificityHuman Immunodeficiency Virus Type 1(HIV 1) p24 protein. No detectable reaction has been observed with HIV-2 or SIV (Simian Immunodeficiency Virus) viral lysates by ELISA or Western blot.
  • Tested applicationsWB, Radioimmunoprecipitation assay, ELISA, ICC/IF, Sandwich ELISAmore details
  • Immunogen

    Tissue, cells or virus corresponding to HIV1 p24. ab9071 raised by immunizing BALB/c mice with purified HIV-1 (Strain IIIB) lysate.

  • General notes

    ab9071 is used to detect HIV-1 core protein in virus infected cells, cell lysates, culture fluid, serum or plasma.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified from serum-free culture supernatant.
  • Primary antibody notesThe antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. Useful as solid phase for capture of p24 in an antigen ELISA format.
  • ClonalityMonoclonal
  • Clone number39/5.4A
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab9071 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 10 µg/ml.

ab9071 exhibits reactivity with viral lysates by Western blot

Radioimmunoprecipitation assay Use at an assay dependent concentration. The antibody immunoprecipitates p24 protein from radiolabeled infected cell extracts.
ELISA Use at an assay dependent concentration.

ab9071 is especially useful as a solid phase adsorbent or detector antibody in an ELISA format.

ICC/IF Use a concentration of 1 - 10 µg/ml. Exhibits reactivity with HIV-1 infected cultures using indirect immunofluorescence.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [39/6.14] to HIV1 p24 (ab9072).

Target

  • RelevanceHIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localizationMembrane
  • Alternative names
    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

Anti-HIV1 p24 antibody [39/5.4A] images

  • All lanes : Anti-HIV1 p24 antibody [39/5.4A] (ab9071) at 1/2000 dilution

    Lane 1 : Mock transfected 293T cells - cell lysate
    Lane 2 : 293T cells transfected with HIV DNA - cell lysate

    Secondary
    HRP conjugated goat anti-mouse IgG
    developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 24,25,41,55 kDa (why is the actual band size different from the predicted?)


    Exposure time : 5 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-HIV1 p24 antibody [39/5.4A] (ab9071)

This product has been referenced in:
  • Rosemary Bastian A  et al. Mechanism of multivalent nanoparticle encounter with HIV-1 for potency enhancement of peptide triazole virus inactivation. J Biol Chem 290:529-43 (2015). Read more (PubMed: 25371202) »
  • Morrison JH  et al. Feline immunodeficiency virus envelope glycoproteins antagonize tetherin through a distinctive mechanism that requires virion incorporation. J Virol 88:3255-72 (2014). WB . Read more (PubMed: 24390322) »

See all 13 Publications for this product

Product Wall

Application Western blot
Loading amount 0.5 µg
Gel Running Conditions Reduced Denaturing (4-12%)
Sample HIV Purified protein (bacteria)
Specification bacteria
Blocking step Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Apr 14 2014

Application Western blot
Loading amount 15 µg
Gel Running Conditions Reduced Denaturing (10)
Sample HIV Cell lysate - whole cell (CEM-CCRF)
Specification CEM-CCRF
Treatment infected with NL.4-3 HIV-1
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

Submitted Feb 17 2015

Thank you for contacting us.

I can confirm that the Anti-HIV1 p24 antibody [39/5.4A] (ab9071) and the Anti-HIV1 p24 antibody [39/6.14] (ab9072) were made by injection of purified HIV-1 (Strain IIIB) lysate into mice.

I hope this infor...

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Thank you for contacting us.

Sorry, we do not know the epitope of this antibody. We do know that it does not appear to react with HIV-2 or SIV antigen.

I hope this information is helpful to you. Please do not hesitate to contact us if...

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Thank you for contacting us.

I have good news and bad news for you:

We unfortunately cannot offer your a sELISA system in which the detection is conjugated either with HRP or Biotin.

The good news is that we indeed have m...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements for one vial of ab63913 and one vial of ab16284 with the order number 1147505.
...

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Thank you for contacting us.

Unfortunately, we do not have a kit for this purpose, but we have two products which may interest you:

http://www.abcam.com/index.html?datasheet=9071 (or use the following: http://www.abcam.com/index.ht...

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Gracias por contactarnos.

Anti-HIV1 p24 [39/5.4A] (ab9071) tiene un precio de 370€ y se encuentra actualmente en stock, por lo que si lo pedís esta tarde o mañana (martes 12) a lo largo del día (antes de las 17 horas...

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Thank you for your participation in Abcam's post complaint survey.

I am sorry that the replacement antibody ab63958 did not work either.

Please let me know what problems you encountered (no bands as before?), and what antibody dilut...

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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"