Overview

  • Product nameAnti-HIV1 p24 antibody [39/6.14]
    See all HIV1 p24 primary antibodies
  • Description
    Mouse monoclonal [39/6.14] to HIV1 p24
  • SpecificityReacts with Human Immunodeficiency Virus Type 1(HIV 1) p24 protein. Cross reacts with Human Immunodeficiency Virus Type 2 and Simian Immunodeficiency Virus (SIV).
  • Tested applicationsSuitable for: ICC/IF, WB, Radioimmunoprecipitation, ELISA, Sandwich ELISAmore details
  • General notes


    The antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. This antibody is useful as solid phase for capture of p24 in an antigen ELISA format

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesPurified from serum-free culture supernatant.
  • Primary antibody notesThe antibody is used to detect HIV 1 core protein in virus infected cells as well as in viral or infected cell lysates. Studies on core antigen synthesis and metabolism can be performed using Western blotting or radioimmunoprecipitation analysis. This antibody is useful as solid phase for capture of p24 in an antigen ELISA format
  • ClonalityMonoclonal
  • Clone number39/6.14
  • IsotypeIgG1
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab9072 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 1 - 10 µg/ml.
WB Use a concentration of 1 - 10 µg/ml.
Radioimmunoprecipitation Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [39/5.4A] to HIV1 p24 (ab9071).

Target

  • RelevanceHIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localizationMembrane
  • Alternative names
    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

Anti-HIV1 p24 antibody [39/6.14] images

  • All lanes : Anti-HIV1 p24 antibody [39/6.14] (ab9072) at 1/1000 dilution

    Lane 1 : Untransfected 293T cells
    Lane 2 : Human 293T cells transfected with HIV proviral DNA

    Secondary
    Goat anti-mouse HRP conjugated antibody
    Developed using the ECL technique

    Performed under reducing conditions.

    Observed band size : 24,45 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes

    See Abreview

References for Anti-HIV1 p24 antibody [39/6.14] (ab9072)

This product has been referenced in:
  • Hazleton JE  et al. Purinergic receptors are required for HIV-1 infection of primary human macrophages. J Immunol 188:4488-95 (2012). ICC/IF ; Human . Read more (PubMed: 22450808) »
  • Throm RE  et al. Efficient construction of producer cell lines for a SIN lentiviral vector for SCID-X1 gene therapy by concatemeric array transfection. Blood 113:5104-10 (2009). WB . Read more (PubMed: 19286997) »

See all 4 Publications for this product

Product Wall

Thank you for contacting us.

I can confirm that the Anti-HIV1 p24 antibody [39/5.4A] (ab9071) and the Anti-HIV1 p24 antibody [39/6.14] (ab9072) were made by injection of purified HIV-1 (Strain IIIB) lysate into mice.

I hope this infor...

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Thank you for contacting us.

I have good news and bad news for you:

We unfortunately cannot offer your a sELISA system in which the detection is conjugated either with HRP or Biotin.

The good news is that we indeed have m...

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I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacements for one vial of ab63913 and one vial of ab16284 with the order number 1147505.
...

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Thank you for contacting us.

I can confirm that anti-HIV1 p24 antibodies ab9071 and ab9072 are suitable for use in performing sandwich ELISAs to detect HIV1 p24. They recognise different epitopes of the protein and have been used previously ...

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Thank you for the interest in these products. The antibodies mentioned Anti-HIV1 p24 antibody [39/6.14] (ab9072) and Goat polyclonal Secondary Antibody to Mouse IgG - H&L (HRP) (ab6789) have both been tested separately using ELISA and should be compati...

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Thank you for your enquiry. We do not know the epitope recognized by these two antibodies. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (transfected 293T)
Specification transfected 293T
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted Feb 13 2006

I was unable to get an image of the blot used by the researcher who tested ab9072 unfortunately; I'm very sorry for the inconvenience. I was able to find out the band was wide and hence could be measured as 24-25kDa in size. To better determine the ...

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Thank you for your email and I'm sorry to hear that you are experiencing difficulty with the latest vial that you received. As this is the third vial of ab9072 that you tried and the previous two vials worked well for you, my hunch is that there was a ...

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We have not had this antibody sequenced. It may react with HIV-2 p27, but this is all the information that we have at this time.

1-10 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"