Anti-HIV1 p24 antibody [5] (ab63958)

Overview

  • Product nameAnti-HIV1 p24 antibody [5]
    See all HIV1 p24 primary antibodies
  • Description
    Mouse monoclonal [5] to HIV1 p24
  • SpecificityReacts with HIV1 native p24 as well rp24 expressed in bacteria and Cos-7 cells. Reacts with rNef of HIV1 subtype B, C and A.
  • Tested applicationsSuitable for: ELISA, WB, ICC/IF, Radioimmunoprecipitationmore details
  • Species reactivity
    Reacts with HIV1.
  • Immunogen

    Synthetic peptide:

    TPQDLNTMLNTVGGH

    , corresponding to amino acids 48-62 of HIV1 HXB2 p24.

Properties

Associated products

Applications

Our Abpromise guarantee covers the use of ab63958 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/25000.
WB 1/10000. Predicted molecular weight: 24 kDa.
ICC/IF Use at an assay dependent dilution.
Radioimmunoprecipitation Use at an assay dependent dilution.

Target

  • RelevanceHIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localizationMembrane
  • Alternative names
    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

Anti-HIV1 p24 antibody [5] images

  • All lanes : Anti-HIV1 p24 antibody [5] (ab63958) at 1/1000 dilution

    Lane 1 : Hela cells infected with HIV1 (4 hours)
    Lane 2 : Hela cells infected with HIV1 (4 hours)
    Lane 3 : Hela cells infected with HIV1 (4 hours)

    Secondary
    Goat anti-mouse conjugated to HRP at 1/2500 dilution

    Predicted band size : 24 kDa

    This image is courtesy of an anonymous Abreview

    See Abreview

References for Anti-HIV1 p24 antibody [5] (ab63958)

ab63958 has not yet been referenced specifically in any publications.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (Hela cell line)
Loading amount 50 µg
Specification Hela cell line
Gel Running Conditions Reduced Denaturing (15% TrisHCl Criterion gel)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
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Verified customer

Submitted Nov 26 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"