Overview

  • Product nameAnti-HIV1 p24 antibody (HRP)
    See all HIV1 p24 primary antibodies
  • Description
    Goat polyclonal to HIV1 p24 (HRP)
  • ConjugationHRP
  • SpecificityHIV1 p24 antibody (ab20365) targets p24 (predominate p24 band pattern in Western blot). Does not cross-react with human T or B cells. Antiserum is not reactive with human or bovine sera.
  • Tested applicationsSuitable for: ELISA, ICC, WBmore details
  • Immunogen

    Full length native protein (purified native p24 from strain IIIb).

  • General notes

    Purified IgG fraction covalently coupled to a highly purified preparation of horseradish peroxidase (RZ>3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is removed.

Properties

Applications

Our Abpromise guarantee covers the use of ab20365 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/200 - 1/1000.
ICC 1/20 - 1/200.
WB 1/200 - 1/1000. Predicted molecular weight: 56 kDa. Dilution optimised using Chromogenic detection. When using chemiluminescent substrates, the assay will require much less antibody. Depending on the sensitivity of the substrate, the antibody dilution may be 1/10,000 - 1/100,000.

Target

  • RelevanceHIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
  • Cellular localizationMembrane
  • Alternative names
    • CA antibody
    • Capsid protein p24 antibody
    • HIV-1 Gag p24 antibody
    • HIV1gp1 antibody
    • Human immunodeficiency virus 1 antibody
    • Human immunodeficiency virus type 1 p24 antibody
    see all

References for Anti-HIV1 p24 antibody (HRP) (ab20365)

This product has been referenced in:
  • Guth CA & Sodroski J Contribution of PDZD8 to stabilization of the human immunodeficiency virus type 1 capsid. J Virol 88:4612-23 (2014). ELISA . Read more (PubMed: 24554657) »

See 1 Publication for this product

Product Wall

Application ELISA
Sample HIV Purified protein (purified from bacteria overexpression)
Specification purified from bacteria overexpression
Type Direct
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 25°C
Username

Dr. Shijian Zhang

Verified customer

Submitted Jun 03 2014

Thank you for your inquiry. I know that you said you're using nitrocellulose, and this protocol uses PVDF, but I hope you can adjust it accordingly. http://www.abcam.com/index.html?pageconfig=resource&rid=11456 I hope this information helps. Please ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"