Overview

Description

  • NatureRecombinant
  • SourceE. coli
  • Amino Acid Sequence

    Specifications

    Our Abpromise guarantee covers the use of ab43037 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Biological activityImmunoreactive with all sera of HIV1 infected individuals.
    • Applications

      ELISA

      Western blot

    • Purity> 95 % by SDS-PAGE.
      Organic extraction > S-Sepharose > S-300.
    • FormLiquid
    • Additional Notes

      Immunoreactive with all sera of HIV-1 infected individuals.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

      Preservative: None
      Constituents: 8M Urea, 50mM Tris, pH 7.2

    General info

    • Alternative names
      • CA
      • Capsid protein p24
      • HIV-1 Gag p24
      • HIV1gp1
      • Human immunodeficiency virus 1
      • Human immunodeficiency virus type 1 p24
      see all
    • RelevanceHIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex.
    • Cellular localizationMembrane

    References for HIV1 p24 full length protein (ab43037)

    This product has been referenced in:

    See 1 Publication for this product

    Product Wall

    Unfortunately we are unable to share the specific sequence but it is available in the literature. The protein is derived from group MN. These regions are highly conserved throughout all strains.

    Thank you for contacting us.


    The immune-biological activity of HIV p24 is derived from its linear AA sequences and has no secondary structure related activity. This protein has not been tested for functional activity.

    I hope...

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    You are correct, ab43037 is an antigen, not an antibody, and it is suitable to be coated on an ELISA plate from 100 ng to 10 ug. The dilution buffer that you use does not have to contain urea, as long as you are coating the wells within the range given...

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    ab43037 works well in ELISA at nano to tens of micro gram levels. Thus, dilutions within that range should work sufficiently.

    Thank you for contacting us.

    The laboratory provided the following ranges for ELISA. Using ab43037 protein use 100 ng to 10 ug to coat the plate, detection with ab81433 use at 1/5000-1/10000.

    I hope this information is helpful to ...

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    Thank you for contacting us.
    I have checked some of the HIV1 p24 proteins available in our catalogue with my lab colleagues. Regarding their suitability for ELISPOT, I am afraid it hasn’t been tested in our lab, but due to their suitability in ELI...

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    Thank you for contacting us.

    Unfortunately, we do not have a kit for this purpose, but we have two products which may interest you:

    http://www.abcam.com/index.html?datasheet=9071 (or use the following: http://www.abcam.com/index.ht...

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    You are welcome! Take all the time you need. I look forward to your reply whenever you are ready.

    Thank you for your reply. Unfortunately, I would not recommend the protein ab73768. This is only a fragment of the HIV1 p24 that lacks large parts of the protein such as the N-terminal amino acids (1-76). If the antibodies you would like to test re...

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    Thank you for contacting us. The differences are as follows: ab43036 HTLV I p24 protein: This protein is the full length sequence of HTLV I p24, i.e. an in E. coli derived recombinant protein. The Human T-lymphotropic virus Type I (HTLV-1) is not...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"