• Product nameAnti-HIV1 p55 + p24 + p17 antibody
  • Description
    Rabbit polyclonal to HIV1 p55 + p24 + p17
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with HIV1.
  • Immunogen

    Recombinant protein p55 over-expressed in E.coli using a plasmid carrying the Gag p55 coding region of HIV1 virus.

  • Positive control
    • Extract of MT4 cells infected with HIV1 (LAI strain).



Our Abpromise guarantee covers the use of ab63917 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/2000. Detects bands of approximately 17, 24 and 55 kDa.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. p55 is digested by HIV1 protease into intermediate products p41 and p15. p41 is further digested into matrix protein p17 and capsid protein p24. Likewise, p15 is further digested into nucleocapsid protein p7 and to p6 and p1.
    • Alternative names
      • CA antibody
      • Capsid protein p24 antibody
      • Gag antibody
      • Gag polyprotein antibody
      • HIV1 matrix protein p17 antibody
      • HIV1 Pr55Gag antibody
      • Human immunodeficiency virus 1 p17 antibody
      • Human immunodeficiency virus 1 p55 antibody
      • Human immunodeficiency virus type 1 p24 antibody
      • MA antibody
      • Matrix protein antibody
      • Matrix protein p17 antibody
      • Pr55 antibody
      • Pr55Gag antibody
      see all

    Anti-HIV1 p55 + p24 + p17 antibody images

    • All lanes : Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution

      Lane 1 : Extract of MT4 cells
      Lane 2 : Extract of MT4 cells infected with HIV1 (LAI strain)

      Observed band size : 17,24,55 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 39 kDa,45 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-HIV1 p55 + p24 + p17 antibody (ab63917)

    This product has been referenced in:
    • Trabalza A  et al. Enhanced central nervous system transduction with lentiviral vectors pseudotyped with RVG/HIV-1gp41 chimeric envelope glycoproteins. J Virol 88:2877-90 (2014). Read more (PubMed: 24371049) »
    • Gunzenhäuser J  et al. A Quantitative Approach to Evaluate the Impact of Fluorescent Labeling on Membrane-Bound HIV-Gag Assembly by Titration of Unlabeled Proteins. PLoS One 9:e115095 (2014). WB . Read more (PubMed: 25493438) »

    See all 3 Publications for this product

    Product Wall

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Western blot
    Sample Human Cell lysate - whole cell (HeLa)
    Loading amount 20000 cells
    Specification HeLa
    Treatment Transfected with plasmids expressing HIV gag
    Gel Running Conditions Reduced Denaturing (12)
    Blocking step Milk as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

    David Carpentier

    Verified customer

    Submitted Jan 07 2010