The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/2000. Detects a band of approximately 17,24,55 kDa.
Use at an assay dependent concentration.
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA.
p55 is digested by HIV1 protease into intermediate products p41 and p15. p41 is further digested into matrix protein p17 and capsid protein p24. Likewise, p15 is further digested into nucleocapsid protein p7 and to p6 and p1.
Capsid protein p24 antibody
Gag polyprotein antibody
HIV1 matrix protein p17 antibody
HIV1 Pr55Gag antibody
Human immunodeficiency virus 1 p17 antibody
Human immunodeficiency virus 1 p55 antibody
Human immunodeficiency virus type 1 p24 antibody
Matrix protein antibody
Matrix protein p17 antibody
Western blot - Anti-HIV1 p55 + p24 + p17 antibody (ab63917)
All lanes : Anti-HIV1 p55 + p24 + p17 antibody (ab63917) at 1/2000 dilution
Lane 1 : Extract of MT4 cells Lane 2 : Extract of MT4 cells infected with HIV1
Santos da Silva E et al. The Envelope Cytoplasmic Tail of HIV-1 Subtype C Contributes to Poor Replication Capacity through Low Viral Infectivity and Cell-to-Cell Transmission. PLoS One11:e0161596 (2016).
Read more (PubMed: 27598717) »
Valera MS et al. The HDAC6/APOBEC3G complex regulates HIV-1 infectiveness by inducing Vif autophagic degradation. Retrovirology12:53 (2015).
Read more (PubMed: 26105074) »