Overview

  • Product nameAnti-HIV1 Rev antibody [Rev-6]
    See all HIV1 Rev primary antibodies
  • Description
    Mouse monoclonal [Rev-6] to HIV1 Rev
  • Specificityab85529 detects the Rev protein of HIV1. No cross reactivity has been observed with non HIV1 Rev proteins.
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    HIV
  • Immunogen

    HIV1 Rev recombinant protein (clade B, HXB-3 isolate) with an N terminal His tag, expressed in bacteria.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.05% Glycerol, 0.1M Sodium citrate, 0.05mM Tris, pH neutral
  • Concentration information loading...
  • PurityProtein A purified
  • Purification notesPurified from supernatants of hybridoma cell cultures by affinity chromatography based on Protein A.
  • ClonalityMonoclonal
  • Clone numberRev-6
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab85529 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/250 - 1/500. Predicted molecular weight: 13 kDa.
ELISA 1/100 - 1/200.

Target

  • RelevanceHIV1 expresses the trans-activator protein Rev which is required for virus growth as it promotes the localisation and expression of viral RNA in the cytoplasm. Rev is a model experimental system for studying nuclear transport. The HIV1 Rev protein as viral protein is expressed early in the virus life cycle and thus may be important targets for the immune control of HIV1 infection.
  • Alternative names
    • Human immunodeficiency virus 1 Rev antibody
    • Protein Rev antibody
    • Regulator of expression of viral proteins antibody
    • Rev antibody

Anti-HIV1 Rev antibody [Rev-6] images



  • Predicted band size : 13 kDa

    Image from Wong R et al., Retrovirology. 2011 Jun 17;8:47.Fig 8.; doi:10.1186/1742-4690-8-47; 17 June, 2011, Retrovirology, 8:47

References for Anti-HIV1 Rev antibody [Rev-6] (ab85529)

This product has been referenced in:
  • Wong RW  et al. Digoxin suppresses HIV-1 replication by altering viral RNA processing. PLoS Pathog 9:e1003241 (2013). WB . Read more (PubMed: 23555254) »
  • Wong RW  et al. Characterization of novel inhibitors of HIV-1 replication that function via alteration of viral RNA processing and rev function. Nucleic Acids Res 41:9471-83 (2013). Human . Read more (PubMed: 23945945) »

See all 4 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (HEK293 transfected with HIV-1Rev expression plasmi)
Loading amount 10 µg
Specification HEK293 transfected with HIV-1Rev expression plasmi
Gel Running Conditions Reduced Denaturing (15%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 18°C
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Submitted Jan 08 2013

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"