Anti-HIV1 Reverse Transcriptase antibody [8C4D7] (ab87398)

Overview

  • Product nameAnti-HIV1 Reverse Transcriptase antibody [8C4D7]
    See all HIV1 Reverse Transcriptase primary antibodies
  • Description
    Mouse monoclonal [8C4D7] to HIV1 Reverse Transcriptase
  • SpecificityBinds to recombinant Reverse Transcriptase HIV1 (IIIB).
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with HIV1,(IIIB).
  • Immunogen

    Purified full length recombinant HIV1 Reverse Transcriptase produced in E. coli Expression system.

Properties

  • FormLyophilised:Reconstitute with distilled water
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified IgG, >95% pure by SDS-PAGE analysis. Not tested for sterility or endotoxin content.
  • ClonalityMonoclonal
  • Clone number8C4D7
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab87398 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: 1/1000 (when antibody concentration is 1 mg/ml)
    WB: 1/500 - 1/1000 (when antibody concentration is 1 mg/ml).

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
    • Alternative names
      • HIV 1 Reverse transcriptase antibody
      • HIV1 RT antibody
      • Human Immunodeficiency virus 1 antibody
      • p51 RT antibody
      • p66 RT antibody
      • Reverse transcriptase p51 subunit antibody
      • Reverse transcriptase p66 subunit antibody
      see all

    References for Anti-HIV1 Reverse Transcriptase antibody [8C4D7] (ab87398)

    This product has been referenced in:

    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"