Overview

  • Product nameAnti-HIV1 Reverse Transcriptase antibody
    See all HIV1 Reverse Transcriptase primary antibodies
  • Description
    Rabbit polyclonal to HIV1 Reverse Transcriptase
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with HIV1.
  • Immunogen

    HIV1 Reverse Transcriptase, over-expressed as recombinant protein in E.coli

  • Positive control
    • Extract of MT4 cells infected with HIV1 (LAI strain).

Properties

Applications

Our Abpromise guarantee covers the use of ab63911 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2500. Detects a band of approximately 51, 66 kDa.
ELISA Use at an assay dependent dilution.

Target

  • RelevanceHIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
  • Alternative names
    • HIV 1 Reverse transcriptase antibody
    • HIV1 RT antibody
    • Human Immunodeficiency virus 1 antibody
    • p51 RT antibody
    • p66 RT antibody
    • Reverse transcriptase p51 subunit antibody
    • Reverse transcriptase p66 subunit antibody
    see all

Anti-HIV1 Reverse Transcriptase antibody images

  • All lanes : Anti-HIV1 Reverse Transcriptase antibody (ab63911) at 1/2500 dilution

    Lane 1 : Extract of MT4 cells
    Lane 2 : Extract of MT4 cells infected with HIV-1 (LAI strain)


    Observed band size : 51,66 kDa (why is the actual band size different from the predicted?)
  • Anti-HIV1 Reverse Transcriptase antibody (ab63911) at 1/2500 dilution + Recombinant HIV1 Reverse Transcriptase protein (ab63979) at 0.01 µg

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Exposure time : 90 seconds

References for Anti-HIV1 Reverse Transcriptase antibody (ab63911)

This product has been referenced in:
  • Contreras X  et al. Protein kinase C-delta regulates HIV-1 replication at an early post-entry step in macrophages. Retrovirology 9:37 (2012). WB . Read more (PubMed: 22554282) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"