Anti-HIV1 Reverse Transcriptase antibody (ab63912)

Overview

  • Product nameAnti-HIV1 Reverse Transcriptase antibody
    See all HIV1 Reverse Transcriptase primary antibodies
  • Description
    Guinea pig polyclonal to HIV1 Reverse Transcriptase
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with HIV1.
  • Immunogen

    HIV1 Reverse Transcriptase, over-expressed as recombinant protein in E.coli.

  • Positive control
    • Extract of MT4 cells infected with HIV-1 (LAI strain).

Properties

Applications

Our Abpromise guarantee covers the use of ab63912 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
ELISA
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/2500. Detects bands of approximately 51 and 66 kDa.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • RelevanceHIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
    • Alternative names
      • HIV 1 Reverse transcriptase antibody
      • HIV1 RT antibody
      • Human Immunodeficiency virus 1 antibody
      • p51 RT antibody
      • p66 RT antibody
      • Reverse transcriptase p51 subunit antibody
      • Reverse transcriptase p66 subunit antibody
      see all

    Anti-HIV1 Reverse Transcriptase antibody images

    • All lanes : Anti-HIV1 Reverse Transcriptase antibody (ab63912) at 1/2500 dilution

      Lane 1 : Extract of MT4 cells
      Lane 2 : Extract of MT4 cells infected with HIV-1 (LAI strain)


      Observed band size : 51,66 kDa (why is the actual band size different from the predicted?)

    References for Anti-HIV1 Reverse Transcriptase antibody (ab63912)

    ab63912 has not yet been referenced specifically in any publications.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"