Anti-HIV1 Reverse Transcriptase antibody (ab63912)
Key features and details
- Guinea pig polyclonal to HIV1 Reverse Transcriptase
- Suitable for: WB, ELISA
- Reacts with: Human immunodeficiency virus
- Isotype: IgG
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Overview
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Product name
Anti-HIV1 Reverse Transcriptase antibody
See all HIV1 Reverse Transcriptase primary antibodies -
Description
Guinea pig polyclonal to HIV1 Reverse Transcriptase -
Host species
Guinea pig -
Tested applications
Suitable for: WB, ELISAmore details -
Species reactivity
Reacts with: Human immunodeficiency virus -
Immunogen
HIV1 Reverse Transcriptase, over-expressed as recombinant protein in E.coli.
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Positive control
- Extract of MT4 cells infected with HIV-1 (LAI strain).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.09% Sodium azide
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab63912 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2500. Detects a band of approximately 51, 66 kDa.
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ELISA |
Use at an assay dependent concentration.
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Notes |
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WB
1/2500. Detects a band of approximately 51, 66 kDa. |
ELISA
Use at an assay dependent concentration. |
Target
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Relevance
HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied. -
Database links
- SwissProt: P03367 Human immunodeficiency virus
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Alternative names
- HIV 1 Reverse transcriptase antibody
- HIV1 RT antibody
- Human Immunodeficiency virus 1 antibody
see all
Images
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All lanes : Anti-HIV1 Reverse Transcriptase antibody (ab63912) at 1/2500 dilution
Lane 1 : Extract of MT4 cells
Lane 2 : Extract of MT4 cells infected with HIV-1 (LAI strain)
Observed band size: 51,66 kDa why is the actual band size different from the predicted?
ab63912 detects bands of 66 kDa and 51 kDa corresponding to the two subunits of HIV1 reverse transcriptase.
Datasheets and documents
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Datasheet download
References (0)
ab63912 has not yet been referenced specifically in any publications.