The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution.
WB: 1/2500. Detects bands of approximately 51 and 66 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid.
It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
HIV 1 Reverse transcriptase antibody
HIV1 RT antibody
Human Immunodeficiency virus 1 antibody
p51 RT antibody
p66 RT antibody
Reverse transcriptase p51 subunit antibody
Reverse transcriptase p66 subunit antibody
Western blot - HIV1 Reverse Transcriptase antibody (ab63912)
All lanes : Anti-HIV1 Reverse Transcriptase antibody (ab63912) at 1/2500 dilution
Lane 1 : Extract of MT4 cells Lane 2 : Extract of MT4 cells infected with HIV-1 (LAI strain)