Directly conjugated antibodies for immunofluorescence

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Anti-HIV1 Reverse Transcriptase antibody [AS35] (Biotin) (ab16162)

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Overview

  • Product nameAnti-HIV1 Reverse Transcriptase antibody [AS35] (Biotin)
    See all HIV1 Reverse Transcriptase primary antibodies
  • Description
    Mouse monoclonal [AS35] to HIV1 Reverse Transcriptase (Biotin)
  • ConjugationBiotin
  • Tested applicationsELISA, IPmore details
  • Immunogen

    Recombinant HIV1 Reverse Transcriptase

  • EpitopeRecognizes an epitope on HIV-RT that is different from AS34 epitope.

Properties

Applications

Our Abpromise guarantee covers the use of ab16162 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
IP
  • Application notesELISA: This antibody has been used as solution phase ab at 2 µg/ml in a two site ELISA with AS34. Typically the ELISA has a working range of 2-500 ng/ml and a sensitivity of <1 ng/ml HIV-RT.
    IP: This antibody has been used to immunoprecipitate > 90% HIV-RT in solution using a double-antibody method. Immunoprecipitation has also been accomplished using Protein G agarose.

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceHIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
    • Alternative names
      • HIV 1 Reverse transcriptase antibody
      • HIV 1 RT antibody
      • HIV RT antibody
      • HIV1 RT antibody
      • Human Immunodeficiency virus 1 antibody
      • Immuno Deficiency Virus Reverse Transcriptase antibody
      • p51 RT antibody
      • p66 RT antibody
      • Reverse transcriptase p51 subunit antibody
      • Reverse transcriptase p66 subunit antibody
      see all

    References for Anti-HIV1 Reverse Transcriptase antibody [AS35] (Biotin) (ab16162)

    This product has been referenced in:
    • Midgley AR & Hepburn MR Use of the double-antibody method to separate antibody bound from free ligand in radioimmunoassay. Methods Enzymol 70:266-74 (1980). Read more (PubMed: 7421592) »

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