Anti-HIV1 Reverse Transcriptase antibody [AS35] (Biotin) (ab16162)
- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-HIV1 Reverse Transcriptase antibody [AS35] (Biotin)
See all HIV1 Reverse Transcriptase primary antibodies -
DescriptionMouse monoclonal [AS35] to HIV1 Reverse Transcriptase (Biotin)
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Host speciesMouse
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ConjugationBiotin
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Tested applicationsSuitable for: ELISA, IPmore details
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Immunogen
Recombinant HIV1 Reverse Transcriptase
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EpitopeRecognizes an epitope on HIV-RT that is different from AS34 epitope.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.08% Sodium Azide
Constituents: Ca2+ & Mg2+ free Dulbecco's PBS -
Concentration information loading... -
PurityProtein G purified
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Purification notesAntibody Purity > 95 % by SDS PAGE
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ClonalityMonoclonal
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Clone numberAS35
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IsotypeIgG1
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab16162 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | ||
| IP |
Application notes
ELISA: This antibody has been used as solution phase ab at 2 µg/ml in a two site ELISA with AS34. Typically the ELISA has a working range of 2-500 ng/ml and a sensitivity of <1 ng/ml HIV-RT.
IP: This antibody has been used to immunoprecipitate > 90% HIV-RT in solution using a double-antibody method. Immunoprecipitation has also been accomplished using Protein G agarose.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
IP: This antibody has been used to immunoprecipitate > 90% HIV-RT in solution using a double-antibody method. Immunoprecipitation has also been accomplished using Protein G agarose.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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RelevanceHIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid. It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
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Alternative names
- HIV 1 Reverse transcriptase antibody
- HIV1 RT antibody
- Human Immunodeficiency virus 1 antibody
see all
Protocols
References
This product has been referenced in:
- Midgley AR & Hepburn MR Use of the double-antibody method to separate antibody bound from free ligand in radioimmunoassay. Methods Enzymol 70:266-74 (1980). Read more (PubMed: 7421592) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"