The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: This antibody has been used as solution phase ab at 2 µg/ml in a two site ELISA with AS34. Typically the ELISA has a working range of 2-500 ng/ml and a sensitivity of <1 ng/ml HIV-RT.
IP: This antibody has been used to immunoprecipitate > 90% HIV-RT in solution using a double-antibody method. Immunoprecipitation has also been accomplished using Protein G agarose.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid.
It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.
HIV 1 Reverse transcriptase antibody
HIV1 RT antibody
Human Immunodeficiency virus 1 antibody
p51 RT antibody
p66 RT antibody
Reverse transcriptase p51 subunit antibody
Reverse transcriptase p66 subunit antibody
References for Anti-HIV1 Reverse Transcriptase antibody [AS35] (Biotin) (ab16162)
This product has been referenced in:
Midgley AR & Hepburn MR Use of the double-antibody method to separate antibody bound from free ligand in radioimmunoassay. Methods Enzymol70:266-74 (1980).
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