The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: This antibody has been used as solution phase ab at 2 µg/ml in a two site ELISA with AS34. Typically the ELISA has a working range of 2-500 ng/ml and a sensitivity of <1 ng/ml HIV-RT.
IP: This antibody has been used to immunoprecipitate > 90% HIV-RT in solution using a double-antibody method. Immunoprecipitation has also been accomplished using Protein G agarose.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
HIV1 Reverse Transcriptase is an RNA-dependent DNA polymerase of HIV1 (AIDS virus), subtype B origin. It is responsible for the production of a double stranded DNA copy of the single stranded RNA genome that is contained in the HIV1 virus particle and primed by the host cell lysine-tRNA which partly unfolds and anneals to the 5' end of the viral genomic RNA. This is extended by the polymerase function of RT to give a DNA-RNA hybrid.
It also has RNaseH activity which degrades the RNA component of this hybrid once it has been copied.