Overview

  • Product nameAnti-HIV1 tat antibody [1]
    See all HIV1 tat primary antibodies
  • Description
    Mouse monoclonal [1] to HIV1 tat
  • SpecificityThis antibody reacts with the rTat consensus sequences of HIV1 subtypes A (DPVDPNLE), B (EPVDPRLE) and C (EPVDPNLE).
  • Tested applicationsSuitable for: Radioimmunoprecipitation, IHC-P, IHC-Fr, ELISA, WBmore details
  • Immunogen

    Recombinant full length protein (Human)

  • EpitopeMapped to amino acids 2-9 (EPVDPRLE - B subtype consensus).
  • Positive control
  • General notesAlso known as clone 02-001.

Properties

Applications

Our Abpromise guarantee covers the use of ab42359 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Radioimmunoprecipitation Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration. PubMed: 20651230
ELISA 1/500 - 1/2000.
WB 1/500 - 1/2000.

Target

  • RelevanceHIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
  • Cellular localizationNuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
  • Alternative names
    • p14 antibody
    • Protein Tat antibody
    • Transactivating regulatory protein antibody

Anti-HIV1 tat antibody [1] images

  • Lane 1 : Anti-HIV1 tat antibody [1] (ab42359)
    Lane 2 :

    Lane 1 : Recombinant HIV1 Tat (B subtype)
    Lane 2 : Protein size marker

References for Anti-HIV1 tat antibody [1] (ab42359)

This product has been referenced in:
  • Pereira LA  et al. MYB elongation is regulated by the nucleic acid binding of NF?B p50 to the intronic stem-loop region. PLoS One 10:e0122919 (2015). Read more (PubMed: 25853889) »
  • Vandergaast R  et al. Generation of West Nile virus infectious clones containing amino acid insertions between capsid and capsid anchor. Viruses 6:1637-53 (2014). WB . Read more (PubMed: 24721788) »

See all 10 Publications for this product

Product Wall

Thank you for your responce. I have been unable to find data showing HIV1 tat in the cytoplasm. I am sorry that I could not be of more help.  I would recommend using positive and negative controls to ensure that your results are accurate. I would lik...

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Thank you for contacting Abcam.

I am sorry to hear of this issue with our product, especially after your earlier work seemed to have exciting implications. This product is very well mapped and I am surprised to hear of the results that you h...

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Thank you for your reply. The information that you have sent and the tissue that you are staining on would seem to explain the membrane staining nicely. I certainly do appreciate your finding this for me and will add this information to our notes. ...

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Thank you for contacting us. I am sorry to hear you are experiencing difficulties with one of our products. We take product complaints very seriously, and investigate every product that we feel may not be performing correctly. I would like to gather ...

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