Overview

  • Product name
  • Description
    Chicken polyclonal to HIV1 tat
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    This antibody may react to various subtypes of HIV 1 tat protein.
  • Immunogen

    Full length HIV 1 tat protein (Human).

  • Positive control
  • General notes
    During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200 µL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

Properties

Applications

Our Abpromise guarantee covers the use of ab14029 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500. Predicted molecular weight: 12 kDa.

Target

  • Relevance
    HIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
  • Cellular localization
    Nuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
  • Alternative names
    • p14 antibody
    • Protein Tat antibody
    • Transactivating regulatory protein antibody

References

ab14029 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Thank you for your enquiry. Below please find the protocol used for blotting with this antibody. The antibody dilution was 1:2000, and recombinant fusion protein used as the antigen. I hope this information helps. Please do not hesitate to cont...

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I'm sorry to hear you are having a problem with ab14029, thank you for taking the time to fill in the questionnaire, it is very useful. I would like to suggest the following modifications to your protocol: -fix your cells for 5-10min in ice cold ac...

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We don't know the viral strain and the source of virus. However, this antibody should recognize tat from various stains since this is a polyclonal antibody against the full length protein.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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