The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application notesELISA: 1/500. Use Streptavidin (HRP) conjugated detection reagent.
Flow Cyt: 1/200. Use Streptavidin-FITC conjugated detection reagent.
WB: 1/1000. Predicted molecular weight: 14 kDa. Use Streptavidin (HRP) conjugated detection reagent.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceHIV1 tat (Transactivator of Transcription) protein is a pleiotropic factor that induces a broad range of biological effects in numerous cell types. At the HIV promoter, tat is a powerful transactivator of gene expression which acts by inducing chromatin remodeling and by recruiting elongation-competent transcriptional complexes on to the viral LTR.
Cellular localizationNuclear. Upon stimulation with EDN1, it is exported from the nucleus to the perinuclear region.
Protein Tat antibody
Transactivating regulatory protein antibody
References for Anti-HIV1 tat antibody (Biotin) (ab43015)
has not yet been referenced specifically in any publications.
Publishing research using ab43015? Please let us know so that we can cite the reference in this datasheet.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"