Products:Microbiology >> Organism >> Virus >> RNA Virus >> ssRNA positive strand virus >> HIV
If your product does not perform as described on this datasheet, we will refund or replace your product...
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Phoned because this product did not work in ELISA or WB against recombinant protein. |
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Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products. |
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Testing discount codefor ab43014 in ICC/IF. |
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DISCOUNT CODE: ********** |
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I would like to get this antibody, but need to make sure it will recognize gp 120 in the human macrophage lysate that I am working with. Since it says "does not reacts with human...", please let me know. Another thing is we use HIV BAL not IIIB strain, would this be a problem with detection? I would also like same information for ab43014 HIV tat antibody. Thanks and have a great afternoon, |
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ANSWER: |
Thank you for your patience. I have enquired at the originator of the product but unfortunately, this product has not been tested against human macrophages and HIV strain BAL. Therefore, we do not know if it will be suitable for your research. If you decide to go ahead and purchase this product, we encourage you to submit an Abreview via the online product datasheet. We always encourage customers to send their results back to us, whether positive or negative, and we make all product information available to researchers. To find out more about our Abreview system, please see the following URL: http://www.abcam.com/abreviews I am sorry I can't be of more assistance in this occasion but please do not hesitate to contact us if you need any more advice or information. |
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
All lanes : Anti-HIV1 tat antibody - ChIP Grade (ab43014) at 1/2000 dilution
Lane 1 : Jurkat whole cell lysate 0 hours after infection
Lane 2 : Jurkat whole cell lysate 2 hours after infection
Lane 3 : Jurkat whole cell lysate 4 hours after infection
Lane 4 : Jurkat whole cell lysate 6 hours after infection
Lane 5 : Jurkat whole cell lysate 8 hours after infection
Lane 6 : Jurkat whole cell lysate 10 hours after infection
Lysates/proteins at 25 µg per lane.
Secondary
HRP Goat anti-rabbit IgG polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 14 kDa
Observed band size : 14 kDa
Jurkat cells were pNL4.3 infected and samples taken at 2 hour intervals for blotting.
This image is courtesy of an anonymous Abreview
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