Anti-HIVEP2 antibody [2418C2a] (ab70599)
Key features and details
- Mouse monoclonal [2418C2a] to HIVEP2
- Suitable for: WB
- Reacts with: Recombinant fragment
- Isotype: IgG1
Overview
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Product name
Anti-HIVEP2 antibody [2418C2a] -
Description
Mouse monoclonal [2418C2a] to HIVEP2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Recombinant fragment -
Immunogen
Recombinant fragment of human HIVEP2 from near the C terminus.
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Positive control
- Recombinant human HIVEP2.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 0.1136% Dibasic monohydrogen sodium phosphate, 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, 0.0204% Monobasic dihydrogen potassium phosphate -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab70599 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
Clonality
Monoclonal -
Clone number
2418C2a -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70599 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Notes |
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WB: Use at an assay dependent dilution. Detects bands of approximately 35 and 39 kDa (predicted molecular weight: 269 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
This protein specifically binds to the DNA sequence 5'-GGGACTTTCC-3' which is found in the enhancer elements of numerous viral promoters such as those of SV40, CMV, or HIV1. In addition, related sequences are found in the enhancer elements of a number of cellular promoters, including those of the class I MHC, interleukin-2 receptor, somatostatin receptor II, and interferon-beta genes. It may act in T-cell activation. -
Tissue specificity
Expressed in brain and skeletal muscle. -
Sequence similarities
Contains 4 C2H2-type zinc fingers. -
Cellular localization
Nucleus. - Information by UniProt
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Alternative names
- HIV EP2 antibody
- HIV-EP2 antibody
- HIVEP2 antibody
see all
Images
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Anti-HIVEP2 antibody [2418C2a] (ab70599) + immunising recombinant protein
Predicted band size: 269 kDa
Observed band size: 35,39 kDa why is the actual band size different from the predicted?
The molecular weight of the bands on the western blot does not correspond to the molecular weight of the natural protein because only a fragment of the protein was used.
Datasheets and documents
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Datasheet download
References (1)
ab70599 has been referenced in 1 publication.
- Bash-Imam Z et al. Translational reprogramming of colorectal cancer cells induced by 5-fluorouracil through a miRNA-dependent mechanism. Oncotarget 8:46219-46233 (2017). PubMed: 28515355