• Product name
  • Description
    Rabbit polyclonal to HJURP
  • Tested applications
    Suitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HJURP aa 650-700. The exact sequence is proprietary.
    Database link: NP_060880.3

  • Positive control
    • HeLa, 293T lysates
  • General notes
    Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.


  • Form
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    Purified using an epitope specific to HJURP immobilized on solid support.
  • Clonality
  • Isotype
  • Research areas


Our Abpromise guarantee covers the use of ab100800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000.


  • Relevance
    Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.
  • Cellular localization
    Nucleus; nucleolus. Centromere. Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage.
  • Database links
  • Alternative names
    • 14-3-3-associated AKT substrate antibody
    • FAKTS antibody
    • fetal liver expressing gene 1 antibody
    • Fetal liver-expressing gene 1 protein antibody
    • FLEG1 antibody
    • hFLEG1 antibody
    • HJURP antibody
    • Holliday junction recognition protein antibody
    • Up-regulated in lung cancer 9 antibody
    • URLC9 antibody
    see all

Anti-HJURP antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling HJURP with ab100800 at 1/1000 (1µg/mg). Detection: DAB.
  • All lanes : Anti-HJURP antibody (ab100800) at 0.1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate at 50 µg
    Lane 2 : HeLa Whole Cell Lysate at 15 µg
    Lane 3 : HeLa Whole Cell Lysate at 5 µg
    Lane 4 : 293T Whole Cell Lysate at 50 µg

    Predicted band size : 84 kDa

    Exposure time : 3 minutes
  • ab100800 at 1 µg/ml staining HJURP in HeLa cell lysate immunoprecipitated using ab100800 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds

References for Anti-HJURP antibody (ab100800)

This product has been referenced in:
  • Bravaccini S  et al. New biomarkers to predict the evolution of in situ breast cancers. Biomed Res Int 2014:159765 (2014). IHC ; Human . Read more (PubMed: 25243117) »

See 1 Publication for this product

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Thank you for your enquiry and interest in our products. We are happy to reassure our customers that all of our products are covered by our Abpromise, which guarantees that the product will work in the applications and species specified on the datashee...

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