Overview

  • Product nameAnti-HJURP antibody
    See all HJURP primary antibodies
  • Description
    Rabbit polyclonal to HJURP
  • Tested applicationsSuitable for: WB, IP, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human HJURP aa 650-700. The exact sequence is proprietary.
    Database link: NP_060880.3

  • Positive control
    • HeLa, 293T lysates
  • General notesAntibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: Tris citrate/phosphate, pH 7-8
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • Purification notesPurified using an epitope specific to HJURP immobilized on solid support.
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab100800 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 84 kDa.
IP Use at 2-5 µg/mg of lysate.
IHC-P 1/500 - 1/2000.

Target

  • RelevanceCentromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions.
  • Cellular localizationNucleus; nucleolus. Centromere. Note: Localizes in centromeres during late telophase and early G1, when CENPA nucleosomes are assembled. Localizes to nucleolus during S phase, nucleolus site being often related to storage.
  • Database links
  • Alternative names
    • 14-3-3-associated AKT substrate antibody
    • FAKTS antibody
    • fetal liver expressing gene 1 antibody
    • Fetal liver-expressing gene 1 protein antibody
    • FLEG1 antibody
    • hFLEG1 antibody
    • HJURP antibody
    • Holliday junction recognition protein antibody
    • Up-regulated in lung cancer 9 antibody
    • URLC9 antibody
    see all

Anti-HJURP antibody images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human skin carcinoma tissue labelling HJURP with ab100800 at 1/1000 (1µg/mg). Detection: DAB.
  • All lanes : Anti-HJURP antibody (ab100800) at 0.1 µg/ml

    Lane 1 : HeLa Whole Cell Lysate at 50 µg
    Lane 2 : HeLa Whole Cell Lysate at 15 µg
    Lane 3 : HeLa Whole Cell Lysate at 5 µg
    Lane 4 : 293T Whole Cell Lysate at 50 µg


    Predicted band size : 84 kDa


    Exposure time : 3 minutes
  • ab100800 at 1 µg/ml staining HJURP in HeLa cell lysate immunoprecipitated using ab100800 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Exposure time: 30 seconds

References for Anti-HJURP antibody (ab100800)

This product has been referenced in:
  • Bravaccini S  et al. New biomarkers to predict the evolution of in situ breast cancers. Biomed Res Int 2014:159765 (2014). IHC ; Human . Read more (PubMed: 25243117) »

See 1 Publication for this product

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