Anti-HL60 antibody [IPO-M6] (ab24093)

Overview

  • Product nameAnti-HL60 antibody [IPO-M6]
  • Description
    Mouse monoclonal [IPO-M6] to HL60
  • SpecificityReacts with human leukemia cell line HL60 and immuno-precipitates with two proteins with MW of 48 and 52 kDa. ab24093 does not stain B cell lines Daudy, PHS, Namalwa, RPMI-1788 and T-cell lines CCRF-HSB2, Jurkat and Molt-4.
  • Tested applicationsSuitable for: IHC-Fr, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human): HL60 cell line.

  • General notes


    This antibody can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia were ab24093 positive. The antigen, defined with this mAb, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages were also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B-ALL were ab24093 negative.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.05% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • PurityProtein G purified
  • Primary antibody notesThis antibody can be applied for staining of monocytes and up to 10 % of lymphocytes from peripheral blood of healthy donors. Blast cells of patients with AMMonL (M5 following FAB classification), AMMonL (M4) and hairy cells leukemia were ab24093 positive. The antigen, defined with this mAb, is particularly expressed on blood cells from patients with infectious mononucleosis and CLL. Histiocytes and macrophages were also positive. Malignant cells from patients with AML (M1 and M2), T-ALL, B-ALL were ab24093 negative.
  • ClonalityMonoclonal
  • Clone numberIPO-M6
  • MyelomaP3x63-Ag8.653
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab24093 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • RelevanceHL-60 cells are used as an in vitro model of acute promyelocytic leukaemia and for differentiation and apoptosis studies. The HL60 cell line was established in 1977 from a patient with acute myeloid leukaemia. The cells largely resemble promyelocytes but can be induced to differentiate terminally in vitro. Some reagents cause HL60 cells to differentiate to granulocyte-like cells, others to monocyte/macrophage-like cells. The HL60 cell genome contains an amplified c-myc proto-oncogene; c-myc mRNA levels are correspondingly high in undifferentiated cells but decline rapidly following induction of differentiation.
  • Alternative names
    • human leukemia cell line HL60 antibody

References for Anti-HL60 antibody [IPO-M6] (ab24093)

ab24093 has not yet been referenced specifically in any publications.

Product Wall

Thank you for contacting us. The antibody ab24093 has not yet been tested in ICC and do not know how well it might work. But I think there would be a good chance of it being successful as it works well in frozen sections (similar protocol) and flow ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"