Anti-HLA A2 antibody [BB7.2] (ab74674)

Overview

  • Product name
    Anti-HLA A2 antibody [BB7.2]
    See all HLA A2 primary antibodies
  • Description
    Mouse monoclonal [BB7.2] to HLA A2
  • Tested applications
    Suitable for: IP, Flow Cytmore details
    Unsuitable for: WB
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HLA-A2 solubilised by papain

  • Epitope
    The C-terminus of alpha-2 helix and a turn on one of the underlying beta strands.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Clonality
    Monoclonal
  • Clone number
    BB7.2
  • Isotype
    IgG2b
  • Light chain type
    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab74674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
  • Application notes
    Is unsuitable for WB.
  • Target

    • Relevance
      Involved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein.
    • Cellular localization
      Membrane; Single-pass type I membrane protein.
    • Database links
    • Alternative names
      • Antigen presenting molecule antibody
      • HLA A antibody
      • HLA A histocompatibility type antibody
      • HLA antibody
      • HLA class I antibody
      • HLA class I histocompatibility antigen A 2 alpha chain antibody
      • HLA class I histocompatibility antigen A2 alpha chain antibody
      • HLA-A antibody
      • HLAA antibody
      • Human leukocyte antigen antibody
      • Leucocyte antigen class I antibody
      • Leukocyte antigen class I A antibody
      • Major histocompatibility complex class I A antibody
      • Major histocompatibility complex class I antibody
      • MHC class I antigen [precursor] antibody
      • MHC class I antigen A antibody
      • MHC class I antigen A*2 antibody
      • MHC class I antigen A2 antibody
      • MHC class I antigen HLA A antibody
      • MHC class I antigen HLA A heavy chain antibody
      • MHC leukocyte antigen antibody
      see all

    Anti-HLA A2 antibody [BB7.2] images

    • Overlay histogram showing Raji cells stained with ab74674 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74674, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Raji cells used under the same conditions.

      Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

    References for Anti-HLA A2 antibody [BB7.2] (ab74674)

    ab74674 has not yet been referenced specifically in any publications.

    Product Wall

    Code de remise :xxxxxxx
    Date d’expiration : 08/04/12

    Je suis ravie que vous souhaitiez participer a notre offre et tester ab74674 en western blot. Le code de réduction correspondant est xxxxxxxxx. Ce code de réductio...

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    Merci de votre intérêt pour ab74674. A ma connaissance, cet anticorps n’a pas encore été testé en western blot et nous n’offrons pas d’échantillon gratuit à des fins de tests prél...

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    Thank you for contacting us.

    Unfortunately we have not determined which specific HLA-A2 allele(s) this clone binds. However, it is clear from the paper you kindly provided that the clone binds HLA-A201. Unfortunately, we currently do not have ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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