Anti-HLA A2 antibody [BB7.2] (ab74674)


  • Product nameAnti-HLA A2 antibody [BB7.2]
    See all HLA A2 primary antibodies
  • Description
    Mouse monoclonal [BB7.2] to HLA A2
  • Tested applicationsSuitable for: IP, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HLA-A2 solubilised by papain

  • EpitopeThe C-terminus of alpha-2 helix and a turn on one of the underlying beta strands.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by protein-A affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberBB7.2
  • IsotypeIgG2b
  • Light chain typekappa
  • Research areas


Our Abpromise guarantee covers the use of ab74674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
Flow Cyt Use 2µg for 106 cells. ab170192-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • RelevanceInvolved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein.
  • Cellular localizationMembrane; Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • Antigen presenting molecule antibody
    • HLA A antibody
    • HLA A histocompatibility type antibody
    • HLA antibody
    • HLA class I antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A2 alpha chain antibody
    • HLA-A antibody
    • HLAA antibody
    • Human leukocyte antigen antibody
    • Leucocyte antigen class I antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • Major histocompatibility complex class I antibody
    • MHC class I antigen [precursor] antibody
    • MHC class I antigen A antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A2 antibody
    • MHC class I antigen HLA A antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC leukocyte antigen antibody
    see all

Anti-HLA A2 antibody [BB7.2] images

  • Overlay histogram showing Raji cells stained with ab74674 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74674, 2µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed Raji cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

References for Anti-HLA A2 antibody [BB7.2] (ab74674)

ab74674 has not yet been referenced specifically in any publications.

Product Wall

Code de remise :xxxxxxx
Date d’expiration : 08/04/12

Je suis ravie que vous souhaitiez participer a notre offre et tester ab74674 en western blot. Le code de réduction correspondant est xxxxxxxxx. Ce code de réductio...

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Merci de votre intérêt pour ab74674. A ma connaissance, cet anticorps n’a pas encore été testé en western blot et nous n’offrons pas d’échantillon gratuit à des fins de tests prél...

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Thank you for contacting us.

Unfortunately we have not determined which specific HLA-A2 allele(s) this clone binds. However, it is clear from the paper you kindly provided that the clone binds HLA-A201. Unfortunately, we currently do not have ...

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