Overview

  • Product name
    Anti-HLA A2 antibody [BB7.2] (FITC)
    See all HLA A2 primary antibodies
  • Description
    Mouse monoclonal [BB7.2] to HLA A2 (FITC)
  • Conjugation
    FITC. Ex: 493nm, Em: 528nm
  • Specificity
    Ab27728 recognises the human HLA A2 histocompatability antigen.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Papain solubilised HLA A2 (Human).

  • Epitope
    The epitope recognised by this antibody has been studied extensively and would appear to include the carboxy terminus of the alpha 2 helix and a turn on one of the underlying beta strands.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide.
    Constituents: 1% BSA, PBS.
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Ab27728 was purified from tissue culture supernatant, and prepared by affinity chromatography.
  • Clonality
    Monoclonal
  • Clone number
    BB7.2
  • Myeloma
    NS1
  • Isotype
    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab27728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10. Use 10 µl of the suggested working dilution to label 106 cells or 100µl whole blood.



ab91368-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.

Target

  • Relevance
    Involved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein.
  • Cellular localization
    Membrane; Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • Antigen presenting molecule antibody
    • HLA A antibody
    • HLA A histocompatibility type antibody
    • HLA antibody
    • HLA class I antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A2 alpha chain antibody
    • HLA-A antibody
    • HLAA antibody
    • Human leukocyte antigen antibody
    • Leucocyte antigen class I antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • Major histocompatibility complex class I antibody
    • MHC class I antigen [precursor] antibody
    • MHC class I antigen A antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A2 antibody
    • MHC class I antigen HLA A antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC leukocyte antigen antibody
    see all

Images

  • Ab27728, undiluted or at a dilution of 1/10, staining HLA A2 in human peripheral blood monocytes by FACS.

References

This product has been referenced in:
  • Li D  et al. Engineering chimeric human and mouse major histocompatibility complex (MHC) class I tetramers for the production of T-cell receptor (TCR) mimic antibodies. PLoS One 12:e0176642 (2017). Flow Cyt . Read more (PubMed: 28448627) »
  • Straetemans T  et al. Recurrence of melanoma following T cell treatment: continued antigen expression in a tumor that evades T cell recruitment. Mol Ther 23:396-406 (2015). Read more (PubMed: 25363716) »

See all 6 Publications for this product

Customer reviews and Q&As

Thank you for contacting us.

Unfortunately we have not determined which specific HLA-A2 allele(s) this clone binds. However, it is clear from the paper you kindly provided that the clone binds HLA-A201. Unfortunately, we currently do not have ...

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Thank you for filling out the questionnaire. Your protocol looks good. Do you incubate the cells with the antibody in the dark? FITC is sensitive to photo-bleaching. The only other recommendation I can make would be to try BSA instead of FBS for blocki...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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