• Product nameAnti-HLA A2 antibody [BB7.2] (FITC)
    See all HLA A2 primary antibodies
  • Description
    Mouse monoclonal [BB7.2] to HLA A2 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • SpecificityAb27728 recognises the human HLA A2 histocompatability antigen.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Papain solubilised HLA A2 (Human).

  • EpitopeThe epitope recognised by this antibody has been studied extensively and would appear to include the carboxy terminus of the alpha 2 helix and a turn on one of the underlying beta strands.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide.
    Constituents: 1% BSA, PBS.
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesAb27728 was purified from tissue culture supernatant, and prepared by affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberBB7.2
  • MyelomaNS1
  • IsotypeIgG2b
  • Research areas


Our Abpromise guarantee covers the use of ab27728 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/10. Use 10 µl of the suggested working dilution to label 106 cells or 100µl whole blood.

ab91368-Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.


  • RelevanceInvolved in the presentation of foreign antigens to the immune system. Dimer of alpha chain and a beta chain (beta-2-microglobulin). Interacts with human herpesvirus 8 MIR1 protein.
  • Cellular localizationMembrane; Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • Antigen presenting molecule antibody
    • HLA A antibody
    • HLA A histocompatibility type antibody
    • HLA antibody
    • HLA class I antibody
    • HLA class I histocompatibility antigen A 2 alpha chain antibody
    • HLA class I histocompatibility antigen A2 alpha chain antibody
    • HLA-A antibody
    • HLAA antibody
    • Human leukocyte antigen antibody
    • Leucocyte antigen class I antibody
    • Leukocyte antigen class I A antibody
    • Major histocompatibility complex class I A antibody
    • Major histocompatibility complex class I antibody
    • MHC class I antigen [precursor] antibody
    • MHC class I antigen A antibody
    • MHC class I antigen A*2 antibody
    • MHC class I antigen A2 antibody
    • MHC class I antigen HLA A antibody
    • MHC class I antigen HLA A heavy chain antibody
    • MHC leukocyte antigen antibody
    see all

Anti-HLA A2 antibody [BB7.2] (FITC) images

  • Ab27728, undiluted or at a dilution of 1/10, staining HLA A2 in human peripheral blood monocytes by FACS.

References for Anti-HLA A2 antibody [BB7.2] (FITC) (ab27728)

This product has been referenced in:
  • Li D  et al. Engineering chimeric human and mouse major histocompatibility complex (MHC) class I tetramers for the production of T-cell receptor (TCR) mimic antibodies. PLoS One 12:e0176642 (2017). Flow Cyt . Read more (PubMed: 28448627) »
  • Straetemans T  et al. Recurrence of melanoma following T cell treatment: continued antigen expression in a tumor that evades T cell recruitment. Mol Ther 23:396-406 (2015). Read more (PubMed: 25363716) »

See all 6 Publications for this product

Product Wall

Thank you for contacting us.

Unfortunately we have not determined which specific HLA-A2 allele(s) this clone binds. However, it is clear from the paper you kindly provided that the clone binds HLA-A201. Unfortunately, we currently do not have ...

Read More

Thank you for filling out the questionnaire. Your protocol looks good. Do you incubate the cells with the antibody in the dark? FITC is sensitive to photo-bleaching. The only other recommendation I can make would be to try BSA instead of FBS for blocki...

Read More