Anti-HLA B27 antibody [HLA-ABC-m3] (FITC) (ab23840)

Overview

  • Product nameAnti-HLA B27 antibody [HLA-ABC-m3] (FITC)
    See all HLA B27 primary antibodies
  • Description
    Mouse monoclonal [HLA-ABC-m3] to HLA B27 (FITC)
  • ConjugationFITC. Ex: 493nm, Em: 528nm
  • Specificityab23840 is a cytotoxic antibody which detects HLA B27 alloantigen.
  • Tested applicationsSuitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Immune complex precipitated from HLA B27 positive cell line (Bordin) by anti HLA-antibody and Staphylococcal Protein A.

  • General notes


    ab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: 0.2% BSA, PBS, pH 7.4
  • Concentration information loading...
  • Purification notesab23840 is a purified IgG prepared by ion exchange chromatography.
  • Primary antibody notesab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
  • ClonalityMonoclonal
  • Clone numberHLA-ABC-m3
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab23840 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use 10µl for 106 cells. ab91362-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration. PubMed: 18776939

Target

  • RelevanceInvolved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells.
  • Cellular localizationSingle-pass type 1 membrane protein
  • Database links
  • Alternative names
    • HLAB antibody
    • Leukocyte antigen class I B antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex class I B antibody
    • SPDA1 antibody
    see all

References for Anti-HLA B27 antibody [HLA-ABC-m3] (FITC) (ab23840)

This product has been referenced in:
  • de Jong MA  et al. TNF-alpha and TLR agonists increase susceptibility to HIV-1 transmission by human Langerhans cells ex vivo. J Clin Invest 118:3440-3452 (2008). ICC/IF ; Human . Read more (PubMed: 18776939) »
  • Orr K  et al. Utilization of commercial antisera and flow cytometry in HLA-B27 typing. Cytometry 18:17-20 (1994). Read more (PubMed: 8082482) »

See all 3 Publications for this product

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The number of smaples can we worked out from the information on the datasheet, 250ul of antibody is provided. The amountof tess in can do will very much depend on the dilution of antibody that you use, which may r...

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Thank you very much for sending the images. It really helps us understand the problem. I can see from the protocol sent that peripheral blood mononuclear cells were used for the ICC assay. It is a very mixed heterogeneous population, containing differe...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"