FITC Anti-HLA B27 antibody [HLA-ABC-m3] (ab23840)
Key features and details
- FITC Mouse monoclonal [HLA-ABC-m3] to HLA B27
- Suitable for: Flow Cyt
- Reacts with: Human
- Conjugation: FITC. Ex: 493nm, Em: 528nm
- Isotype: IgG2a
Overview
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Product name
FITC Anti-HLA B27 antibody [HLA-ABC-m3]
See all HLA B27 primary antibodies -
Description
FITC Mouse monoclonal [HLA-ABC-m3] to HLA B27 -
Host species
Mouse -
Conjugation
FITC. Ex: 493nm, Em: 528nm -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length protein corresponding to Human HLA B27.
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General notes
ab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituents: PBS, 0.2% BSA -
Concentration information loading...
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Purification notes
ab23840 is a purified IgG prepared by ion exchange chromatography. -
Primary antibody notes
ab23840 reacts with the peripheral blood lymphocytes of 47/47 individuals conventionally typed as HLA B27 + and precipitates cell surface molecules of 43 and 12 kD, corresponding to the HLA Class 1 heavy chain and beta 2 microglobulin. -
Clonality
Monoclonal -
Clone number
HLA-ABC-m3 -
Isotype
IgG2a -
Research areas
Associated products
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab23840 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
Flow Cyt |
Use at an assay dependent concentration.
ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Notes |
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Flow Cyt
Use at an assay dependent concentration. ab91362 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
Target
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Relevance
Involved in the presentation of foreign antigens to the immune system. HLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. -
Cellular localization
Single-pass type 1 membrane protein -
Database links
- Entrez Gene: 3106 Human
- Omim: 142830 Human
- SwissProt: P03989 Human
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Alternative names
- HLAB antibody
- Leukocyte antigen class I B antibody
- Lymphocyte antigen antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (5)
ab23840 has been referenced in 5 publications.
- Tilley MA et al. Perivascular adipose tissue mediated aortic reactivity data: Female lean and obese Zucker rats. Data Brief 29:105290 (2020). PubMed: 32140508
- Chen LG et al. Therapeutic targeting of liver cancer with a recombinant DNA vaccine containing the hemagglutinin-neuraminidase gene of Newcastle disease virus via apoptotic-dependent pathways. Oncol Lett 12:3344-3350 (2016). Flow Cyt . PubMed: 27900002
- de Jong MA et al. TNF-alpha and TLR agonists increase susceptibility to HIV-1 transmission by human Langerhans cells ex vivo. J Clin Invest 118:3440-3452 (2008). ICC/IF ; Human . PubMed: 18776939
- Orr K et al. Utilization of commercial antisera and flow cytometry in HLA-B27 typing. Cytometry 18:17-20 (1994). PubMed: 8082482
- Trapani JA et al. Description of a mouse monoclonal anti-HLA-B27 antibody HLA-ABC-m3. Hum Immunol 7:205-16 (1983). PubMed: 6604046