Anti-HLA B7 antibody [EPR2623(2)] (ab109089)

Overview

  • Product nameAnti-HLA B7 antibody [EPR2623(2)]
    See all HLA B7 primary antibodies
  • Description
    Rabbit monoclonal [EPR2623(2)] to HLA B7
  • Tested applicationsSuitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity
    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in the extracellular domain of Human HLA B7.

  • Positive control
    • Jurkat, HL60, TF1, and Ramos cell lysates; Human tonsil tissue.
  • General notes

    This product is a recombinant rabbit monoclonal antibody.

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
  • PurityTissue culture supernatant
  • ClonalityMonoclonal
  • Clone numberEPR2623(2)
  • IsotypeIgG
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab109089 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 40 kDa.
IHC-P 1/250 - 1/500. Antigen retrieval is recommended.
  • Application notesIs unsuitable for Flow Cyt,ICC or IP.
  • Target

    • RelevanceHLA B belongs to the HLA class I heavy chain paralogues. This class I molecule is a heterodimer consisting of a heavy chain and a light chain (beta 2 microglobulin). The heavy chain is anchored in the membrane. Class I molecules play a central role in the immune system by presenting peptides derived from the endoplasmic reticulum lumen. They are expressed in nearly all cells. The heavy chain is approximately 45 kDa. Hundreds of HLA B alleles have been described.
    • Cellular localizationCell Membrane, single pass type I membrane protein.
    • Database links
    • Alternative names
      • Abacavir hypersensitivity, susceptibility to antibody
      • Drug-induced liver injury due to flucloxacillin antibody
      • HLA B antibody
      • HLA class I histocompatibility antigen B 7 alpha chain antibody
      • HLA class I histocompatibility antigen, B-13 alpha chain antibody
      • HLA class I histocompatibility antigen, B-14 alpha chain antibody
      • HLA class I histocompatibility antigen, B-15 alpha chain antibody
      • HLA class I histocompatibility antigen, B-18 alpha chain antibody
      • HLA class I histocompatibility antigen, B-27 alpha chain antibody
      • HLA class I histocompatibility antigen, B-35 alpha chain antibody
      • HLA class I histocompatibility antigen, B-37 alpha chain antibody
      • HLA class I histocompatibility antigen, B-38 alpha chain antibody
      • HLA class I histocompatibility antigen, B-40 alpha chain antibody
      • HLA class I histocompatibility antigen, B-41 alpha chain antibody
      • HLA class I histocompatibility antigen, B-42 alpha chain antibody
      • HLA class I histocompatibility antigen, B-44 alpha chain antibody
      • HLA class I histocompatibility antigen, B-45 alpha chain antibody
      • HLA class I histocompatibility antigen, B-46 alpha chain antibody
      • HLA class I histocompatibility antigen, B-47 alpha chain antibody
      • HLA class I histocompatibility antigen, B-48 alpha chain antibody
      • HLA class I histocompatibility antigen, B-49 alpha chain antibody
      • HLA class I histocompatibility antigen, B-50 alpha chain antibody
      • HLA class I histocompatibility antigen, B-51 alpha chain antibody
      • HLA class I histocompatibility antigen, B-52 alpha chain antibody
      • HLA class I histocompatibility antigen, B-53 alpha chain antibody
      • HLA class I histocompatibility antigen, B-54 alpha chain antibody
      • HLA class I histocompatibility antigen, B-55 alpha chain antibody
      • HLA class I histocompatibility antigen, B-56 alpha chain antibody
      • HLA class I histocompatibility antigen, B-57 alpha chain antibody
      • HLA class I histocompatibility antigen, B-58 alpha chain antibody
      • HLA class I histocompatibility antigen, B-59 alpha chain antibody
      • HLA class I histocompatibility antigen, B-67 alpha chain antibody
      • HLA class I histocompatibility antigen, B-7 alpha chain antibody
      • HLA class I histocompatibility antigen, B-73 alpha chain antibody
      • HLA class I histocompatibility antigen, B-78 alpha chain antibody
      • HLA class I histocompatibility antigen, B-8 alpha chain antibody
      • HLA class I histocompatibility antigen, B-81 alpha chain antibody
      • HLA class I histocompatibility antigen, B-82 alpha chain antibody
      • HLAB antibody
      • Leukocyte antigen class I B antibody
      • Lymphocyte antigen antibody
      • Major histocompatibility complex class I B antibody
      • MHC class I antigen B 7 antibody
      • Synovitis, chronic, susceptibility to antibody
      see all

    Anti-HLA B7 antibody [EPR2623(2)] images

    • All lanes : Anti-HLA B7 antibody [EPR2623(2)] (ab109089) at 1/1000 dilution

      Lane 1 : Jurkat Ramos cell lysate
      Lane 2 : HL60 cell lysate
      Lane 3 : TF1 cell lysate
      Lane 4 : Ramos cell lysate

      Lysates/proteins at 10 µg per lane.


      Predicted band size : 40 kDa
    • ab109089 at 1/250 dilution staining HLA B7 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue.

    References for Anti-HLA B7 antibody [EPR2623(2)] (ab109089)

    ab109089 has not yet been referenced specifically in any publications.

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    Thank you for your reply and sorry for the delay in my responce.

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    I understand your concerns and it is regrettable the results have not been successful from this replacement vial aswell.

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    Thank you for taking the time to contact us. Your message has been forwarded to me as my colleague Claudia is no longer in the office. I have now taken on this case.

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    Thank you for contacting us and your interest in our products.

    Having searched through the antibodies we have against the targets you have mentioned (HLA B7 and B27) we do not have any antibodies which are specific for only one subtype. For e...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"