Anti-HLA class 2 DQ6 antibody [4i147] (ab42130)

Overview

  • Product nameAnti-HLA class 2 DQ6 antibody [4i147]
  • Description
    Mouse monoclonal [4i147] to HLA class 2 DQ6
  • Specificityab42130 reacts to HLA Class 2 Antigen DQ6. Additional crossreactivity occurs with related proteins. Specificity was determined at a 1:10 dilution by the microcytotoxicity test under standard NIH conditions.
  • Tested applicationsSuitable for: Flow Cyt, Microcytotoxicity testing, Functional Studiesmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HLA class 2 antigen DQ6 (Human)

  • General notes


    Cell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Add glycerol to a final volume of 40% for extra stability and aliquot. Store at -20°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: None
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • PurityAscites
  • Primary antibody notesCell death will occur in any test well where the HLA cell surface antigen is recognized by its matched anti-HLA antibody. Live lymphocytes indicate a negative reaction. Dead lymphocytes indicate a positive reaction. Limitations: Cell isolation difficulties, contamination of the lymphocyte preparation with red blood cells, monocytes, platelets or granulocytes, cell concentrations outside acceptable levels, bacterial contamination and/or change in pH of antisera may cause erroneous results.
  • ClonalityMonoclonal
  • Clone number4i147
  • IsotypeIgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab42130 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab91545-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
Microcytotoxicity testing Use at an assay dependent concentration.

Add 0.9ml of 1% BSA in PBS to 100µl of 10X antibody stock solution.

Functional Studies Use at an assay dependent concentration.

Cell Typing, Tissue Staining and Chimerism Studies.

References for Anti-HLA class 2 DQ6 antibody [4i147] (ab42130)

ab42130 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"