Anti-HLA Class I A1 A36 antibody [8.L.104] (Biotin) (ab31563)

Overview

  • Product name
    Anti-HLA Class I A1 A36 antibody [8.L.104] (Biotin)
  • Description
    Mouse monoclonal [8.L.104] to HLA Class I A1 A36 (Biotin)
  • Conjugation
    Biotin
  • Tested applications
    Suitable for: Flow Cyt, ICC/IFmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    HLA Class 1 Antigen A1, A36.

  • General notes


    This antibody is suitable for use in the detection of HLA antigens on lymphocytes in whole blood samples and cells isolated from blood or lymphoid tissue.

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause. 

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.1% Sodium Azide.
    Constituents: 40% Glycerol, 1% BSA, PBS.
  • Concentration information loading...
  • Purification notes
    This antibody was purified by Ion Exchange chromatography.
  • Primary antibody notes
    This antibody is suitable for use in the detection of HLA antigens on lymphocytes in whole blood samples and cells isolated from blood or lymphoid tissue.
  • Clonality
    Monoclonal
  • Clone number
    8.L.104
  • Isotype
    IgM
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab31563 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. Use 20µl per test.



ab18396-Mouse monoclonal IgM, is suitable for use as an isotype control with this antibody.
ICC/IF Use at an assay dependent concentration.

Target

  • Relevance
    Human leukocyte antigens are highly polymorphic proteins that are involved in the presentation of antigens to the T cell receptor. There are two classes of HLA antigens, class I (HLA A, HLA B and HLA C) and class II (HLA D).
  • Cellular localization
    Membrane; single pass type I membrane protein.
  • Database links
  • Alternative names
    • Aw36 antibody
    • HLA class I histocompatibility antigen A36 alpha chain antibody
    • MHC class I antigen A*36 antibody

References

ab31563 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

This antibody is an IgM and was purified from mouse ascites using chromatography with affinity and ion-exchange mechanisms. The isotyping was performed with Ouchterlony Double Diffusion method. Additionally, we have tested this antibody and know it to ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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