Anti-HLA Class I A3 antibody [7g7h8] (ab86597)

Overview

  • Product nameAnti-HLA Class I A3 antibody [7g7h8]
    See all HLA Class I A3 primary antibodies
  • Description
    Mouse monoclonal [7g7h8] to HLA Class I A3
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 50 - 150 of Human HLA Class I A3.

  • Positive control
    • This antibody gave a positive signal in the following whole cell lysates: Jurkat; Raji; U937. Abcam recommends using 3% milk as a blocking agent.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • PurityIgG fraction
  • ClonalityMonoclonal
  • Clone number7g7h8
  • MyelomaSp2/0
  • IsotypeIgG1
  • Light chain typekappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab86597 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).

Use 3% milk as a blocking agent.

Target

Anti-HLA Class I A3 antibody [7g7h8] images

  • All lanes : Anti-HLA Class I A3 antibody [7g7h8] (ab86597) at 1 µg/ml

    Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 2 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
    Lane 3 : U937 (Human leukemic monocyte lymphoma cell line) Whole Cell Lysate

    Lysates/proteins at 20 µg per lane.

    Secondary
    Goat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 41 kDa
    Observed band size : 41 kDa


    Exposure time : 20 minutes

References for Anti-HLA Class I A3 antibody [7g7h8] (ab86597)

ab86597 has not yet been referenced specifically in any publications.

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Lab has confirmed that they have not determined the endotoxin level of antibody ab33640 and ab86597. This means we will not bwe able to confirm how the antibodies will perform in cell culture experiments.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"