Anti-HLA Class I antibody [MEM-147] (Phycoerythrin) (ab52451)

Overview

  • Product name
    Anti-HLA Class I antibody [MEM-147] (Phycoerythrin)
    See all HLA Class I primary antibodies
  • Description
    Mouse monoclonal [MEM-147] to HLA Class I (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    ab5451 recognises all human classical MHC Class I molecules in native cell-surface forms (e.g. it recognizes native HLA-A2 in cytofluorometry and immunoprecipitation but not in Western blotting). MHC Class I molecules (MHC Class Ia) are expressed on the surface of all human cell types. ab52451 is positive in Western blotting (non-reducing conditions) only with most HLA-B and HLA-C molecules, but not HLA-A.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    PHA-activated peripheral blood lymphocytes.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 15mM Sodium Azide
    Constituents: 0.2% BSA (high-grade protease free), PBS
  • Concentration information loading...
  • Purity
    >95% by SDS-PAGE
  • Purification notes
    ab52451 was purified by Protein G affinity crimatography prior to conjugation. After conjugation, it was purified by site exclusion chromatography.
  • Clonality
    Monoclonal
  • Clone number
    MEM-147
  • Isotype
    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab52451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use a concentration of 5 µg/ml. ab91357-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

References

ab52451 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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