Anti-HLA Class I antibody [MEM-81] (ab3975)

Overview

  • Product nameAnti-HLA Class I antibody [MEM-81]
    See all HLA Class I primary antibodies
  • Description
    Mouse monoclonal [MEM-81] to HLA Class I
  • SpecificityThe antibody reacts with human classical MHC Class I molecules in native cell-surface forms.
  • Tested applicationsSuitable for: IHC-P, Flow Cytmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation (Human). Leucocytes of a patient suffering with LGL type leukaemia.

  • Positive control
    • IHC-P: human spleen FFPE tissue sections

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesPurified from ascites by protein-A affinity chromatography.
  • ClonalityMonoclonal
  • Clone numberMEM-81
  • IsotypeIgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab3975 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt Use a concentration of 1 µg/ml.

Target

Anti-HLA Class I antibody [MEM-81] images

  • Overlay histogram showing Jurkat cells stained with ab3975 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab3975, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

  • IHC image of ab3975 staining in human spleen formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3975, 10µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

References for Anti-HLA Class I antibody [MEM-81] (ab3975)

ab3975 has not yet been referenced specifically in any publications.

Product Wall

Thank you for your email. Ab2217 is isotype IgG3; ab9090 is isotype IgG1; and ab3975 is isotype IgG1. You may also find the isotype information on our online datasheets. If you have any additional questions, please contact us again.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"