Anti-HLA Class I antibody [W6/32] (ab22432)

Overview

  • Product nameAnti-HLA Class I antibody [W6/32]
    See all HLA Class I primary antibodies
  • Description
    Mouse monoclonal [W6/32] to HLA Class I
  • SpecificityRecognises an antigenic determinant shared among products of the HLA A, B and C loci. The antibody recognises virtually all nucleated human cells. It recognises an antigenic determinant shared among products of the HLA A, B and C loci. Clone W6/32 recognises a conformational epitope, reacting with HLA class I alpha3 and alpha2 domains.
  • Tested applicationsSuitable for: ICC/IF, Flow Cyt, IHC-Fr, IP, ELISAmore details
    Unsuitable for: IHC-P
  • Species reactivity
    Reacts with: Cow, Cat, Human
  • Immunogen

    Tissue/ cell preparation of membrane of tonsil cells (Human).

  • Positive control
    • IHC: Human tonsil tissue slides FACS: human peripheral blood lymphocytes

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • ClonalityMonoclonal
  • Clone numberW6/32
  • MyelomaNS1/1-Ag4-1
  • IsotypeIgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22432 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/100.
Flow Cyt 1/50 - 1/100.

Use 10µl of the suggested working dilution to label 106 cells or 100µl whole blood.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.

See Cabrera CM et al reference.

  • Application notesIs unsuitable for IHC-P.
  • Target

    Anti-HLA Class I antibody [W6/32] images

    • ab22432 at a 1/100 dilution staining unpermeabilized human HeLa cells (cervical carcinoma) by Immunocytochemistry. The cells were paraformaldehyde fixed and blocked with 10% serum and incubated with the antibody for 1 hour. Bound antibody was detected using a Goat anti-mouse TRITC antibody.

      This image is courtesy of an Anonymous Abreview submitted on 12 April 2006.

      See Abreview

    • Staining of human peripheral blood lymphocytes with ab22432.

    References for Anti-HLA Class I antibody [W6/32] (ab22432)

    This product has been referenced in:
    • Daniel D  et al. Pathway of apoptosis induced in Jurkat T lymphoblasts by anti-HLA class I antibodies. Hum Immunol 65:189-99 (2004). Read more (PubMed: 15041157) »
    • Andratschke M  et al. MHC-class I antigen expression on micrometastases in bone marrow of patients with head and neck squamous cell cancer. Anticancer Res 23:1467-71 (2003). Read more (PubMed: 12820411) »

    See all 6 Publications for this product

    Product Wall

    I'm afraid this has not been tested, so we do not know if it is a gamma or kappa light chain. Sorry I couldn't help you more on this occasion,

    Abcam guarantees this product to work in the species/application used in this Abreview.
    Application Immunocytochemistry/ Immunofluorescence
    Sample Human Cell (Cervical carcinoma (HeLa))
    Specification Cervical carcinoma (HeLa)
    Fixative Paraformaldehyde
    Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
    Username

    Abcam user community

    Verified customer

    Submitted Apr 12 2006

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"