Anti-HLA Class I antibody [W6/32] (Phycoerythrin) (ab33257)

Overview

  • Product name
    Anti-HLA Class I antibody [W6/32] (Phycoerythrin)
    See all HLA Class I primary antibodies
  • Description
    Mouse monoclonal [W6/32] to HLA Class I (Phycoerythrin)
  • Conjugation
    Phycoerythrin. Ex: 488nm, Em: 575nm
  • Specificity
    This antibody recognises an antigenic determinant shared among products of the HLA A, B and C loci.
  • Tested applications
    Suitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Cow, Cat, Human, Monkey, Baboon, Cynomolgus monkey, Macaque monkey
  • Immunogen

    Tissue/ cell preparation: purified human tonsil lymphocyte membranes.

  • Positive control
    • Human peripheral blood lymphocytes.
  • General notes
    50 tests will be supplied typically at a volume of 0.5ml at a concentration of 0.1mg/ml (concentration prior to conjugation).

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.09% Sodium Azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    This antibody was purified from tissue culture supernatant.
  • Clonality
    Monoclonal
  • Clone number
    W6/32
  • Myeloma
    NS1/1-Ag4-1
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt
  • Application notes
    Flow Cyt: Use 10µl to label 106 cells or 100µl whole blood.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    References

    This product has been referenced in:
    • Hurton LV  et al. Identifying candidate allogeneic NK-cell donors for hematopoietic stem-cell transplantation based on functional phenotype. Leukemia 24:1059-62 (2010). Read more (PubMed: 20164852) »
    • Daniel D  et al. Pathway of apoptosis induced in Jurkat T lymphoblasts by anti-HLA class I antibodies. Hum Immunol 65:189-99 (2004). Read more (PubMed: 15041157) »

    See all 7 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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