Anti-HLA Class I antibody [W6/32] (Phycoerythrin) (ab43545)


  • Product nameAnti-HLA Class I antibody [W6/32] (Phycoerythrin)
    See all HLA Class I primary antibodies
  • Description
    Mouse monoclonal [W6/32] to HLA Class I (Phycoerythrin)
  • ConjugationPhycoerythrin. Ex: 488nm, Em: 575nm
  • SpecificityThis antibody recognises virtually all nucleated human cells. MHC Class I molecules (MHC Class 1a) are expressed on the surface of all human cell types. We have data to indicate that this antibody may not cross react with Rabbit. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: Flow Cytmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Cow, Cat, Non Human Primates, Baboon, Cynomolgus Monkey, Macaque MonkeyDoes not react with: Rabbit
  • Immunogen

    Tissue/ cell preparation (Human): Membrane of human tonsil cells.

  • EpitopeThis clone (W6/32) recognises a conformational epitope, reacting with HLA class I alpha 3 and alpha 2 domains.
  • Positive control
    • Human peripheral blood lymphocytes.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C.
  • Storage bufferPreservative: 15mM Sodium Azide
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity>95% by SDS-PAGE
  • Purification notesThis antibody was purified by size-exclusion chromatography.
  • ClonalityMonoclonal
  • Clone numberW6/32
  • IsotypeIgG2a
  • Research areas


Our Abpromise guarantee covers the use of ab43545 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt 1/50. ab91363-Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.


References for Anti-HLA Class I antibody [W6/32] (Phycoerythrin) (ab43545)

This product has been referenced in:
  • Torikai H  et al. Toward eliminating HLA class I expression to generate universal cells from allogeneic donors. Blood 122:1341-9 (2013). Flow Cyt ; Human . Read more (PubMed: 23741009) »

See 1 Publication for this product

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Grazie per la sua richesta.

Spero cha ha passato una buona pasqua.

Puo per piacere darci il numero del "lot" o della charica del anticorpo? Si non trove il numero del lot, mi puo dare il numero dell'ordine o la data dell'o...

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Grazie per la vostra richiesta e pazienza. Vi mando le informazioni qui in lingua inglese. Per favore fatemi sapere, se avete bisogno di una traduzione.

We do source this antibody from a collaborating laboratory and I have contacted the labor...

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Thank you for your enquiry. The antibodies that you highlight do not in fact have different specificities. They are all the same clone, same Immunoglobulin class and therefore carry the same epitope recognition. I can tell you that some of your con...

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