Anti-HLA Class I antibody [YTH862.2] (ab33252)
Key features and details
- Rat monoclonal [YTH862.2] to HLA Class I
- Suitable for: Flow Cyt
- Reacts with: Human
- Isotype: IgG2b
Overview
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Product name
Anti-HLA Class I antibody [YTH862.2]
See all HLA Class I primary antibodies -
Description
Rat monoclonal [YTH862.2] to HLA Class I -
Host species
Rat -
Specificity
ab33252 recognises a monomorphic determinant on the human HLA Class I heavy chain. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues. -
Tested applications
Suitable for: Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Tissue, cells or virus corresponding to Human HLA Class I. PHA activated human peripheral blood lymphocytes.
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Epitope
ab33252 reacts with an epitope located in the alpha1 domain of HLA class I molecules. -
Positive control
- Human peripheral blood lymphocytes
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General notes
Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
ab33252 was purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
YTH862.2 -
Isotype
IgG2b -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab33252 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt |
1/50 - 1/100.
Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Notes |
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Flow Cyt
1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.
ab18536 - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
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Target
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Relevance
HLA CLass I is involved in the presentation of foreign antigens to the immune system. -
Cellular localization
Plasma membrane -
Database links
- Entrez Gene: 3105 Human
- Entrez Gene: 3133 Human
- Entrez Gene: 3134 Human
- Entrez Gene: 3135 Human
- Omim: 142800 Human
- SwissProt: O15506 Human
- SwissProt: P04439 Human
- SwissProt: P13747 Human
see all -
Alternative names
- DKFZp686P19218 antibody
- EA1.2 antibody
- EA2.1 antibody
see all
Images
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab33252 has not yet been referenced specifically in any publications.