Anti-HLA Class I antibody [YTH862.2] (ab33252)

Overview

  • Product nameAnti-HLA Class I antibody [YTH862.2]
    See all HLA Class I primary antibodies
  • Description
    Rat monoclonal [YTH862.2] to HLA Class I
  • Specificityab33252 recognises a monomorphic determinant on the human HLA Class I heavy chain. We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
  • Tested applicationsSuitable for: Functional Studies, Flow Cyt, IHC-Fr, IPmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue/ cell preparation: PHA activated human peripheral blood lymphocytes.

  • Epitopeab33252 reacts with an epitope located in the alpha1 domain of HLA class I molecules.
  • Positive control
    • Human peripheral blood lymphocytes
  • General notes

    Fusion Partners: Spleen cells from immunised DA rats were fused with cells of the rat Y3 myeloma cell line.

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage bufferPreservative: 0.09% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • PurityProtein G purified
  • Purification notesab33252 was purified from tissue culture supernatant.
  • ClonalityMonoclonal
  • Clone numberYTH862.2
  • IsotypeIgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab33252 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Functional Studies Use at an assay dependent concentration. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Flow Cyt 1/50 - 1/100. Use 10µl of the suggested working dilution to label 106 cells in 100µl.



ab18536-Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
IP Use at an assay dependent concentration.

Target

Anti-HLA Class I antibody [YTH862.2] images

  • FACS analysis of HLA class I expressing cells in human peripheral blood lymphocytes using ab33252.

References for Anti-HLA Class I antibody [YTH862.2] (ab33252)

ab33252 has not yet been referenced specifically in any publications.

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Thank you for your phone call. As we discussed, the best staining on mouse tissue will be obtained using a primary antibody raised in a species other than mouse in combination with a secondary antibody that has been pre-adsorbed to remove cross-reactiv...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Frozen sections)
Sample Human Tissue sections (skin)
Specification skin
Fixative Formaldehyde
Permeabilization No
Blocking step BSA as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
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Submitted Jun 17 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"