Anti-HLA Class II DRB1 antibody (ab235953)
Key features and details
- Rabbit polyclonal to HLA Class II DRB1
- Suitable for: ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HLA Class II DRB1 antibody
See all HLA Class II DRB1 primary antibodies -
Description
Rabbit polyclonal to HLA Class II DRB1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein corresponding to Human HLA Class II DRB1 aa 1 to the C-terminus.
Database link: Q95IE3 -
Positive control
- WB: A-375 and Raji whole cell lysates. ICC/IF: A-375 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purity >95%. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab235953 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF |
1/50 - 1/200.
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WB |
1/1000 - 1/5000. Predicted molecular weight: 30 kDa.
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Notes |
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ICC/IF
1/50 - 1/200. |
WB
1/1000 - 1/5000. Predicted molecular weight: 30 kDa. |
Target
- Information by UniProt
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Database links
- Entrez Gene: 3123 Human
- Omim: 142857 Human
- SwissProt: P01911 Human
- SwissProt: P01912 Human
- SwissProt: P04229 Human
- SwissProt: P20039 Human
- SwissProt: Q29974 Human
- SwissProt: Q30134 Human
see all -
Alternative names
- 2B1F_HUMAN antibody
- DR1 antibody
- DR16 antibody
see all
Images
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All lanes : Anti-HLA Class II DRB1 antibody (ab235953) at 1/1000 dilution
Lane 1 : A-375 (Human malignant melanoma cell line) whole cell lysate
Lane 2 : Raji (Human Burkitt's lymphoma cell line) whole cell lysate
Secondary
All lanes : Goat polyclonal to rabbit IgG at 1/10000 dilution
Predicted band size: 30 kDa -
A-375 (Human malignant melanoma cell line) cells stained for HLA Class II DRB1 (Green) using ab235953 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab235953 has not yet been referenced specifically in any publications.