Overview

  • Product name
    Anti-HLA Class II DRB1 antibody [DA2]
    See all HLA Class II DRB1 primary antibodies
  • Description
    Mouse monoclonal [DA2] to HLA Class II DRB1
  • Specificity
    HLA-DR beta chain 1. All DR and DP except DR7. Not specific to humans.
  • Tested applications
    Suitable for: Flow Cyt, IHC-Fr, WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Tissue, cells or virus corresponding to HLA Class II DRB1. Raised against the membranes of LKT cells of human origin.

  • Epitope
    1st domain, monomorphic.
  • General notes
    Not cytotoxic.


    This antibody can be used for HLA typing.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: None
    Constituents: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Primary antibody notes
    This antibody can be used for HLA typing.
  • Clonality
    Monoclonal
  • Clone number
    DA2
  • Myeloma
    P3-x63-Ag8
  • Isotype
    IgG1
  • Light chain type
    unknown
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab20184 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
IHC-Fr Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 30 kDa.

Predicted molecular weight: 30 kDa (unprocessed precursor). Blots dimer and beta chain.

ELISA Use at an assay dependent concentration.

Target

References for Anti-HLA Class II DRB1 antibody [DA2] (ab20184)

This product has been referenced in:

See all 2 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
ELISA
Sample
Human Recombinant protein (HLA DR52)
Specification
HLA DR52
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 37°C
Type
Sandwich (Detection)
Username

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Verified customer

Submitted Oct 07 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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