Recombinant Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6148] to HLA Class II DRB1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
Related conjugates and formulations
Overview
-
Product name
Anti-HLA Class II DRB1 antibody [EPR6148]
See all HLA Class II DRB1 primary antibodies -
Description
Rabbit monoclonal [EPR6148] to HLA Class II DRB1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human HLA Class II DRB1 aa 150-250. The exact sequence is proprietary. Also SwissProt: Q29974
Database link: P01911 -
Positive control
- WB: Human spleen, and Ramos lysates. IHC-P: human tonsil tissue. ICC/IF: Raji cells.
-
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 8.80 x 10 -12 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
-
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR6148 -
Isotype
IgG -
Research areas
Associated products
-
Alternative Versions
-
Isotype control
-
Positive Controls
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab133578 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (1) |
1/2000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Use a HRP/AP polymerized secondary antibody. See IHC antigen retrieval protocols For unprified use at 1/100 - 1/250 |
ICC/IF |
1/50.
For unprified use at 1/100 - 1/250 |
|
WB |
1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
|
Notes |
---|
IHC-P
1/2000. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. Use a HRP/AP polymerized secondary antibody. See IHC antigen retrieval protocols For unprified use at 1/100 - 1/250 |
ICC/IF
1/50. For unprified use at 1/100 - 1/250 |
WB
1/1000 - 1/10000. Predicted molecular weight: 29 kDa. |
Target
- Information by UniProt
-
Database links
- Entrez Gene: 3123 Human
- Omim: 142857 Human
- SwissProt: P01911 Human
- SwissProt: P01912 Human
- SwissProt: P04229 Human
- SwissProt: P20039 Human
- SwissProt: Q29974 Human
- SwissProt: Q30134 Human
see all -
Alternative names
- 2B1F_HUMAN antibody
- DR1 antibody
- DR16 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence analysis of Raji (Human Burkitt's lymphoma B lymphocyte) cells labeling HLA Class II DRB1 with Purified ab133578 at 1:50 dilution (2.24 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue sections labeling HLA Class II DRB1 with Purified ab133578 at 1:2000 dilution (0.06 µg/ml). Perform heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0). Purified)ImmunoHistoProbe one step HRP Polymer (ready to use) was used for detection. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
-
All lanes : Anti-HLA Class II DRB1 antibody [EPR6148] (ab133578) at 1/1000 dilution (Purified)
Lane 1 : Human spleen lysates
Lane 2 : Ramos (Human Burkitt's lymphoma B lymphocyte) whole cell lysates
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 29 kDa
Observed band size: 29 kDa -
Immunohistochemical analysis of HLA Class II DRB1 in formalin fixed, paraffin embedded Human tonsil tissue stained with ab133578 at a 1/100 dilution.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-
ab133578 showing positive staining in Diffuse large B-cell lymphoma tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
-
ab133578 showing positive staining in Normal colon tissue.
Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Protocols
Datasheets and documents
-
SDS download
-
Datasheet download
References (4)
ab133578 has been referenced in 4 publications.
- Higashio A et al. Relationships Between Histopathological Findings in the Liver and Prognosis in Patients With Biliary Atresia. Clin Pathol 15:2632010X221132686 (2022). PubMed: 36339922
- Zhao X et al. HLA-A and HLA-DRB1 may play a unique role in ovarian teratoma-associated anti-N-methyl-D-aspartate receptor encephalitis. Reprod Biol Endocrinol 18:107 (2020). PubMed: 33160385
- Bertram KM et al. Identification of HIV transmitting CD11c+ human epidermal dendritic cells. Nat Commun 10:2759 (2019). PubMed: 31227717
- Kanangat S et al. Circulating histocompatibility antigen (HLA) gene products may help differentiate benign from malignant indeterminate pulmonary lesions. Hum Immunol 79:558-563 (2018). WB . PubMed: 29656111