Overview

  • Product name
  • Description
    Rabbit polyclonal to HLA DMB
  • Specificity
    No cross reactivity with other proteins.
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human HLA DMB aa 81-100 (internal sequence).
    Sequence:

    NQKDTLMQRLRNGLQNCATH


    Database link: P28068

  • Positive control
    • Raji, Jurkat, and HUT cell lysates; Human mammary cancer tissue.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191445 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 29 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Relevance
    HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.
  • Cellular localization
    Late endosome membrane; Single-pass type I membrane protein. Lysosome membrane; Single-pass type I membrane protein. Note=Localizes to late endocytic compartment. Associates with lysosome membranes.
  • Database links
  • Alternative names
    • class II antigen HLA-DM beta chain antibody
    • class II histocompatibility antigen antibody
    • Class II histocompatibility antigen M beta chain antibody
    • D6S221E antibody
    • DMB antibody
    • HLA class II histocompatibility antigen DM beta chain antibody
    • HLA-DM histocompatibility type, beta chain antibody
    • M beta chain antibody
    • Major histocompatibility complex class II DM beta antibody
    • MHC class II antigen DMB antibody
    • MHC class II antigen HLA DM beta chain antibody
    • MHC class II HLA DMB antibody
    • OTTHUMP00000029257 antibody
    • Really interesting new gene 7 protein antibody
    • RING 7 antibody
    • RING7 antibody
    see all

Images

  • All lanes : Anti-HLA DMB antibody (ab191445) at 0.5 µg/ml

    Lane 1 : Jurkat cell lysate
    Lane 2 : Jurkat cell lysate
    Lane 3 : Raji cell lysate
    Lane 4 : HUT cell lysate


    Predicted band size : 29 kDa
  • Immunohistochemical analysis of paraffin-embedded Human mammary cancer tissue labeling HLA DMB with ab191445 at 1 μg/ml.

References

ab191445 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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