Overview

  • Product nameAnti-HLA-DOA antibody
    See all HLA-DOA primary antibodies
  • Description
    Mouse polyclonal to HLA-DOA
  • Tested applicationsSuitable for: WBmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Full length HLA-DOA protein (Human)

  • Positive control
    • HLA-DOA transfected cell lysate

Properties

  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 1X PBS, pH 7.2
  • Concentration information loading...
  • PurityProtein A purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab67079 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Detects a band of approximately 30 kDa (predicted molecular weight: 28 kDa). This antibody has only been tested against the transfected cell lysate.

Target

  • RelevanceHLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level.
  • Cellular localizationCell Membrane; Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • HLA class II histocompatibility antigen, DO alpha chain precursor antibody
    • HLA D0 alpha antibody
    • HLA DNA antibody
    • HLA DZA antibody
    • HLADZ antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex, class II, DN alpha antibody
    • Major histocompatibility complex, class II, DO alpha antibody
    • MHC class II antigen DOA antibody
    • MHC DN alpha antibody
    • MHC DZ alpha antibody
    see all

Anti-HLA-DOA antibody images

  • All lanes : Anti-HLA-DOA antibody (ab67079) at 1/500 dilution

    Lane 1 : HLA-DOA transfected 293T cell lysate
    Lane 2 : Non-transfected 293T cell lysate

    Lysates/proteins at 25 µg/ml per lane.

    Secondary
    Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

    Predicted band size : 28 kDa
    Observed band size : 30 kDa (why is the actual band size different from the predicted?)

References for Anti-HLA-DOA antibody (ab67079)

ab67079 has not yet been referenced specifically in any publications.

Product Wall

I think the customer's protocol is correct with regards to the western blot. My only concerns are whether the samples are intact in the solution that is loaded into the gel, and whether the transfer to the blot was successful. I expect the samples are ...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"