• Product nameAnti-HLA-DOA antibody
    See all HLA-DOA primary antibodies
  • Description
    Rabbit polyclonal to HLA-DOA
  • Tested applicationsSuitable for: WB, ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Synthesized peptide derived from an internal region of human HLA-DOA

  • Positive control
    • extracts from COLO cells.


  • FormLiquid
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • PurityImmunogen affinity purified
  • ClonalityPolyclonal
  • IsotypeIgG
  • Research areas


Our Abpromise guarantee covers the use of ab74981 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/1000. Predicted molecular weight: 28 kDa.
ELISA 1/40000.


  • RelevanceHLA-DOA belongs to the HLA class II alpha chain paralogues. HLA-DOA forms a heterodimer with HLA-DOB. The heterodimer, HLA-DO, is found in lysosomes in B cells and regulates HLA-DM-mediated peptide loading on MHC class II molecules. In comparison with classical HLA class II molecules, this gene exhibits very little sequence variation, especially at the protein level.
  • Cellular localizationCell Membrane; Single-pass type I membrane protein.
  • Database links
  • Alternative names
    • HLA class II histocompatibility antigen, DO alpha chain precursor antibody
    • HLA D0 alpha antibody
    • HLA DNA antibody
    • HLA DZA antibody
    • HLADZ antibody
    • Lymphocyte antigen antibody
    • Major histocompatibility complex, class II, DN alpha antibody
    • Major histocompatibility complex, class II, DO alpha antibody
    • MHC class II antigen DOA antibody
    • MHC DN alpha antibody
    • MHC DZ alpha antibody
    see all

Anti-HLA-DOA antibody images

  • All lanes : Anti-HLA-DOA antibody (ab74981) at 1/500 dilution

    Lane 1 : extracts from COLO cells
    Lane 2 : extracts from COLO cells with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.

    Predicted band size : 28 kDa
    Observed band size : 35 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.

References for Anti-HLA-DOA antibody (ab74981)

ab74981 has not yet been referenced specifically in any publications.

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I think the customer's protocol is correct with regards to the western blot. My only concerns are whether the samples are intact in the solution that is loaded into the gel, and whether the transfer to the blot was successful. I expect the samples are ...

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