The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Detects a band of approximately 31 kDa (predicted molecular weight: 31 kDa).
FunctionImportant modulator in the HLA class II restricted antigen presentation pathway by interaction with the HLA-DM molecule in B cells. Modifies peptide exchange activity of HLA-DM.
Sequence similaritiesBelongs to the MHC class II family. Contains 1 Ig-like C1-type (immunoglobulin-like) domain.
Cellular localizationEndosome membrane. Lysosome membrane. Complexes with HLA-DM molecule during intracellular transport and in endosomal/lysosomal compartments. Heterotetramerization is necessary to exit the ER.